Article thumbnail

Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly▿†

By Alexandra Faulds-Pain, Christopher Birchall, Christine Aldridge, Wendy D. Smith, Giulia Grimaldi, Shuichi Nakamura, Tomoko Miyata, Joe Gray, Guanglai Li, Jay X. Tang, Keiichi Namba, Tohru Minamino and Phillip D. Aldridge

Abstract

Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in ΔfljK ΔfljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament

Topics: Physiology and Metabolism
Publisher: American Society for Microbiology
OAI identifier: oai:pubmedcentral.nih.gov:3133132
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.