Article thumbnail

A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides

By Chunjie Xia, Jianguo Zhang, Weidong Zhang and Bo Hu

Abstract

The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi has great potential because filamentous fungi can form pellets during cell growth, and these pellets are much easier to harvest from cell broth. This paper focuses on the cell pelletization process of the oleaginous Mucor circinelloides. We have studied the effect of various cultural conditions on pelletized cell growth and lipid accumulation. This study is the first to report that pH adjustment during cell growth plays a key role in pellet formation of M. circinelloides and describes a handy method by which to induce cell pelletization in submerged fungal cultivation. Our study reveals that cell growth and lipid production are not significantly affected by pelletization and that lipid accumulation is triggered at stressed conditions, such as a high carbon-to-nitrogen ratio and high temperature

Topics: Research
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:3127746
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2009). Aggelis G: Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina. Biomass Bioenergy
  2. (1998). Asghar A: Synthesis of lipids by certain yeast strains grown on whey permeate. J Food Lipids
  3. (2007). Biodiesel from microalgae. Biotechnol Adv
  4. (2009). Biodiesel production from biomass of an oleaginous fungus. Biochem Eng J
  5. (2008). Co-production of lactic acid and chitin using a pelletized filamentous fungus Rhizopus oryzae cultured on cull potatoes and glucose.
  6. (2009). Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem Eng Process
  7. (2000). Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol
  8. (2008). Guebitz GM: Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production. Bioresour Technol
  9. (1982). HM: Interference in determination of ammonia with the hypochlorite alkaline phenol method of berthelot. Anal Chem
  10. (2007). HZ: Microbial oil accumulation and cellulase secretion of the endophytic fungi from oleaginous plants. Ann Microbiol
  11. (2000). Influence of growth temperature on lipid and soluble carbohydrate synthesis by fungi isolated from fellfield soil in the maritime Antarctic. Mycologia
  12. (1992). JL: Lipid accumulation in Rhodotorula glutinis on sugar cane molasses in single-stage continuous culture.
  13. (1992). Lipid formation in the oleaginous mould Entomophthora exitalis grown in continuous culture: effects of growth rate, temperature and dissolved oxygen tension on polyunsaturated fatty acids. Appl Microbiol Biotechnol
  14. Lipids from microbial sources. In Modifying Lipids for Use in Food. Edited by: Gunstone FD. Boca Raton:
  15. (2007). Malic enzyme: the controlling activity for lipid production? Overexpression of malic enzyme in Mucor circinelloides leads to a 2.5-fold increase in lipid accumulation.
  16. (2008). Microbial and algal oils: do they have a future for biodiesel or as commodity oils? Lipid Technol
  17. (2009). Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol
  18. (1991). MR: Effect of carbon/nitrogen ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana.
  19. (2010). Oil accumulation via heterotrophic/ mixotrophic Chlorella protothecoides. Appl Biochem Biotechnol
  20. (1996). Optimization and scale-up of γ-linolenic acid production by Mortierella ramanniana MM 15-1, a high γ-linolenic acid producing mutant.
  21. (1988). Optimization of lipid production in the oleaginous yeast Apiotrichum curvatum in whey permeate. Appl Microbiol Biotechnol
  22. (2010). Papanikolaou S: Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid. Ind Crops Prod
  23. (1986). Production of lipid from starch by a nitrogen-controlled mixed culture of Saccharomycopsis fibuliger and Rhodosporidium toruloides. Appl Microbiol Biotechnol
  24. (2008). QY: High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Applied Microbiol Biotechnol
  25. (2002). Regulation of lipid accumulation in oleaginous microorganisms. Biochem Soc Trans
  26. (2009). Screening of oleaginous yeast strains tolerant to lignocellulose degradation compounds. Appl Biochem Biotechnol
  27. (1995). SG: Production of γ-linolenic acid by Mucor circinelloides and Mucor rouxii with acetic acid as carbon substrate. Biotechnol Lett
  28. (2008). Single cell oil production in solid-state fermentation by Microsphaeropsis sp. from steam-exploded wheat straw mixed with wheat bran. Bioresour Technol
  29. (2007). SL: Studying pellet formation of a filamentous fungus Rhizopus oryzae to enhance organic acid production. Appl Biochem Biotechnol
  30. (1997). TM: Inoculum effects on the production of γ-linolenic acid by the shake culture of Cunninghamella echinulata CCRC 31840. Enzyme Microb Technol
  31. (1996). Two alternative pathways for substrate assimilation by Mucor circinelloides. Folia Microbiol (Praha)
  32. (2010). V: Direct transformation of fungal biomass from submerged cultures into biodiesel. Energy Fuels
  33. (2009). Zhao ZK: Effects of biomass hydrolysis byproducts on oleaginous yeast Rhodosporidium toruloides. Bioresour Technol
  34. (2011). ZK: Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies.