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Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants

By Hussien H. Daffalla, Eltayb Abdellatef, Elsadig A. Elhadi and Mutasim M. Khalafalla

Abstract

The percent study describes the in vitro responses of mature zygotic embryos of Boscia senegalensis to different concentrations (0.0–5.0 mg/L) of 6-benzyladnine (BA), Thidiazuron (TDZ), α-Naphthalene acetic acid (NAA), and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) supplemented on Murashige and Skoog medium (MS). The plant growth regulators (PGRs) were considerably affected the morphogenetic responses. BA produced adventitious shoots through two ways: direct organogenesis and auxiliary shoot formation. Both 2, 4-D and TDZ tend to produce callus, whereas NAA improve the development of embryos to seedlings. Maximum number of shoots/explant (14.8 ± 0.6) was obtained on MS medium supplemented with 3.0 mg/L BA. 67.0% of excised shoots were rooted either on 1/2 MS medium augmented with or without 0.25 mg/L IBA. The highest number of roots (1.2 ± 0.4) and root length (0.5 ± 0.2 cm) was produced on 0.25 mg/L IBA-containing medium. Regenerated plants were successfully acclimatized and transferred to the green house with 70% survival rate. All the plants appeared morphologically uniform with normal growth pattern. A rapid (30 days), efficient and without subculturing protocol for in vitro regeneration of B. senegalensis was developed

Topics: Research Article
Publisher: SAGE-Hindawi Access to Research
OAI identifier: oai:pubmedcentral.nih.gov:3113285
Provided by: PubMed Central

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Citations

  1. (2001). a n ga n dZ .G u o ,“ In vitro propagation of loblolly pine via direct somatic organogenesis from mature cotyledons and hypocotyls,” Plant Growth Regulation,vol.33,no.1,pp.25–31,
  2. (1997). A simple procedure of Gossypium meristem shoot tip culture,”
  3. (2000). a, “Cytokinins and water stress,”
  4. (1989). Alterations of endogenous cytokinins in transgenic plants using a Chimeric lsopentenyl Transferase gene,” Plant Cell,v o l .1 ,p p .
  5. (2004). andS.Hazra,“Effect of thidiazuron in germinating tamarind seedlings,”
  6. (2009). Auxin-cytokinin interactions in the control of shoot branching,”
  7. (2005). Combining Boscia senegalensis Lamarck (Capparaceae) leaves and augmentation of the larval parasitoid Dinarmus basalis Rondani (Hymenoptera: Pteromalidae) for Bruchid control in stored
  8. (2005). Development of adventitious shoots from in vitro grown Cydonia oblonga leaves as influenced by different cytokinins and treatment duration,”
  9. (2009). Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.): a valuable medicinal tree,”
  10. (2003). Duchefa Catalogue—Biochemicals Plant Cell andTissue Culture, Duchefa Biochemie,Haarlem,TheNetherlands,
  11. (1996). e c k ,G .L o g n a y ,E .H a u b r u g e ,M .M a r l i e r ,a n dC .G a s p a r , “Alternativeprotection ofcowpea seeds againstCallosobruchus maculatus (F.)(Coleoptera: Bruchidae) usinghermetic storage alone or
  12. (2004). Ecological correlates of seed desiccation tolerance in tropical African dryland trees,”
  13. (2008). Ecologyofseedgerminationofeightnon-pioneertree species from a tropical seasonalrain forest in southwest
  14. (2005). Effect of cytokinins on in vitro cultured Exacum affine Balf,”
  15. Effects of salt formulations, carbon sources, cytokinins, and auxin on shootorganogenesis from cotyledons of Pinus pinea
  16. (2003). Endogenous auxin and cytokinin contents associated with shoot formation in leaves of pineapple cultured in vitro,”
  17. (1984). Famine that Kills,
  18. (2004). h a t u r v e d i ,M .K .R a z d a n ,a n dS .S .B h o j w a n i ,“
  19. (1995). How useful is the keystone species concept, and can it be applied to Acacia erioloba
  20. (1993). Identification of elemental sulphur (S8)
  21. (2008). In vitro adventitious shoot organogenesis and plant regeneration from seedling explants of Albizia odoratissima L.f. (Benth.),”
  22. (2007). Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) asa model material,” Plant Cell, Tissue and Organ Culture,
  23. (1994). Influence of auxin type and concentration on peanut somatic embryogenesis,”
  24. (1997). K i m ,A .P a s t u s z y n ,D .J .V a n d e r j a g t
  25. (2008). Lost Crops of Africa, Volume III: Fruits, The National Academies Press,
  26. (2002). Mineral Nutrition: Plant Physiology, Sinaver Associates, 2nd edition,
  27. (2000). Plant responses to drought, acclimation, and stress tolerance,”
  28. (2002). Potential of Australian Acacias in combating hunger in semi-arid lands,”
  29. (2004). Recalcitrant seeds,” in Handbook of Seed Physiology: Applications to Agriculture,R
  30. (2008). Ruˇ zi´ ca n dT .I .V u j o v i ´ c, “The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv.
  31. (1991). S a l i h ,A .M .N o u r ,a n dD .B .H a r p e r ,“ C h e m i c a la n d nutritional composition of two famine food sources used in Sudan, mukheit (Boscia senegalensis)a n dm a i k a h( Dobera roxburghi),”
  32. (2009). Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of in vitro raised plantlets,”
  33. (2010). Taah et al., “The effects of different concentrations cytokinins on the in vitro multiplication of plantain
  34. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture,”
  35. (2004). X.ZhangandE.H.Ervin,“Cytokinin-containingseaweed and humic acid extracts associated with creeping bentgrass leaf cytokinins and drought resistance,”