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A “Silent Culture-Negative” Abdominal Aortic Mycotic Aneurysm: Rapid Detection of Bartonella Species Using PCR and High-Throughput Mass Spectrometry

By Matthew Koo, Sheri Manalili, Matthew J Bankowski, Rangarajan Sampath, Steven A Hofstadler and Joseph Koo


A gram-negative, rod-shaped microorganism was detected in a 69-year-old man suffering from chronic back pain but otherwise exhibiting no signs of infection. The bacterium could not be identified using any routine diagnostic modality. A research use only application utilizing PCR and Mass Spectrometry* was performed on nucleic acid extracted from the tissue sample. These studies resulted in the implication of Bartonella quintana as the underlying cause of the infection. B. quintana is not a well-known cause of an abdominal aortic mycotic aneurysm. This article will discuss the B. quintana infection, its diagnosis and treatment, and reinforce the potential of B. quintana as a possible etiology in mycotic aneurysms that show no apparent indications of infection. It will also explore the potential use of polymerase chain reaction detected by electrospray ionization mass spectrometry (PCR/ ESI-MS) to help identify B. quintana in a situation where other conventional methods prove non-informative

Topics: Articles
Publisher: University Clinical, Education & Research Associate (UCERA)
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Provided by: PubMed Central
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