Article thumbnail

Day and Night GSH and MDA Levels in Healthy Adults and Effects of Different Doses of Melatonin on These Parameters

By Shilpa Chakravarty and Syed Ibrahim Rizvi


The pineal secretory product melatonin (chemically, N-acetyl-5-methoxytryptamine) acts as an effective antioxidant and free-radical scavenger and plays an important role in several physiological functions such as sleep induction, immunomodulation, cardiovascular protection, thermoregulation, neuroprotection, tumor-suppression and oncostasis. Membrane lipid-peroxidation in terms of malondialdehyde (MDA) and intracellular glutathione (GSH) is considered to be a reliable marker of oxidative stress. The present work was undertaken to study the modulating effect of melatonin on MDA and GSH in human erythrocytes during day and night. Our observation shows the modulation of these two biomarkers by melatonin, and this may have important therapeutic implications. In vitro dose-dependent effect of melatonin also showed variation during day and night. We explain our observations on the basis of melatonin's antioxidative function and its effect on the fluidity of plasma membrane of red blood cells. Rhythmic modulation of MDA and GSH contents emphasized the role of melatonin as an antioxidant and its function against oxidative stress

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1984). A Manual of Biochemical Methods, Grunne and Stratton,
  2. (2005). A.V.Domanski,E.A.Lapshina,andI.B.Zavodnik,“Oxidative processes induced by tert-butyl hydroperoxide in human red blood cells: chemiluminescence studies,”
  3. (1997). D’Arpa,and M.Morreale,“Reaction of melatonin with lipoperoxyl radicals in phospholipid bilayers,”
  4. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and
  5. (2007). Diurnal variation in glutathione and cysteine redox states in human plasma,”
  6. (2006). Erythrocyte plasma membrane redox system in human aging,”
  7. (1999). Inhibitory effect of melatonin on products of lipid peroxidation resulting from chronic ethanol administration,” Alcohol and Alcoholism,
  8. (2009). Kˇ riv´ akov´ a, A. L´ abajov´ a et al., “Mechanisms participating in oxidative damage of isolated rat hepatocytes,”
  9. (2009). Kynuramines, metabolites of melatonin and other indoles: the resurrection of an almost forgotten class of biogenic amines,”
  10. (2007). Markers of oxidative stress in erythrocytes during aging in humans,”
  11. (1999). Melatonin as an antioxidant in retinal photoreceptors,”
  12. (2006). Melatonin directly scavenges free radicals generated in red blood cells and a cell-free system: chemiluminescence measurements and theoretical calculations,”
  13. (1997). Melatonin in relation to cellular antioxidative defense mechanisms,”
  14. (2008). Melatonin prevents lipid peroxidation in human erythrocytes but augments deterioration of deformability after in vitro oxidative stress,”
  15. (1999). Melatonin protects human red blood cells from oxidative hemolysis: new insights into the radicalscavenging activity,”
  16. (2007). Melatonin treatment improves the antioxidant status and decreases lipid content in brain and liver of rats,”
  17. (1994). Melatonin: a peroxyl radical scavenger more effective than vitamin
  18. (2010). Melatonin: action as antioxidant and potential applications in human disease and aging,”
  19. (2006). Melatonin: nature’s most versatile biological signal?”
  20. (2003). noz-Hoyos, “Melatonin protects against lipid peroxidation and membrane rigidity in erythrocytes from patients undergoing cardiopulmonary bypass surgery,”
  21. (2002). Protective effect of melatonin against cytotoxic actions ofInternational
  22. (2009). Reducing oxidative/nitrosative stress: a newly-discovered genre for melatonin,”
  23. Role of protein -SH groups in redox homeostasis—the erythrocyte as a model system,” Archives of BiochemistryandBiophysics,vol.355,no.2,pp.145–152,1998.
  24. (2008). Therapeutic actions of melatonin in cancer: possible mechanisms,”
  25. (1997). Y.Urata,S.Honma,S.Gotoetal.,“Melatonininducesgammaglutamylcysteine synthetase mediated by activator protein-1 in human vascular endothelial cells,”