Article thumbnail

lpxC and yafS are the Most Suitable Internal Controls to Normalize Real Time RT-qPCR Expression in the Phytopathogenic Bacteria Dickeya dadantii

By Florence Hommais, Ouafa Zghidi-Abouzid, Christine Oger-Desfeux, Emilie Pineau-Chapelle, Frederique Van Gijsegem, William Nasser and Sylvie Reverchon
Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3102694
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2003). A comparison of normalization methods for high density oligonucleotide array data based on variance and bias.
  2. (2001). A new mathematical model for relative quantification in realtime RT-PCR.
  3. (1990). A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
  4. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
  5. (2009). Bacterial DNA topology and infectious disease.
  6. (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–Excel-based tool using pair-wise correlations.
  7. (1992). Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937.
  8. (2007). Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum.
  9. (2003). Exploration, normalization, and summaries of high density oligonucleotide array probe level data.
  10. (2001). Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58.
  11. (1999). Housekeeping genes as internal standards: use and limits.
  12. (2007). Integration of two essential virulence modulating signals at the Erwinia chrysanthemi pel gene promoters: a role for Fis in the growth-phase regulation.
  13. (1989). Isolation and characterisation of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence.
  14. (2007). Modeling the onset of virulence in a pectinolytic bacterium.
  15. (2008). PecS is a global regulator of the symptomatic phase in the phytopathogenic bacterium Erwinia chrysanthemi 3937.
  16. (2002). Potato diseases caused by soft rot erwinias: an overview of pathogenesis.
  17. (2005). Siderophoremediated upregulation of Arabidopsis ferritin expression in response to Erwinia chrysanthemi infection.
  18. (2010). Systematic targeted mutagenesis of the MarR/SlyA family members of Dickeya dadantii 3937 reveals a role for MfbR in the modulation of virulence gene expression in response to acidic pH.
  19. (2007). The DNA nucleoid-associated protein Fis coordinates the expression of the main virulence genes in the phytopathogenic bacterium Erwinia chrysanthemi. Mol Microbiol 66: 1474–1490. Reference for Expression Normalization in Bacteria PLoS
  20. (2008). The GacA global regulator is required for the appropriate expression of Erwinia chrysanthemi 3937 pathogenicity genes during plant infection.
  21. (2009). The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.
  22. (2009). The transcription unit architecture of the Escherichia coli genome.
  23. (2010). Toward a quantitative modeling of the synthesis of the pectate lyases, essential virulence factors in Dickeya dadantii.
  24. (2005). Transfer of Pectobacterium chrysanthemi
  25. (2004). Vacuolar H+-translocating inorganic pyrophosphatase (Vpp1) marks partial aleurone cell fate in cereal endosperm development.
  26. (2009). Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae.