Article thumbnail

Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

By Haidong Tan, Li Fu and Masaharu Seno


With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation

Topics: Article
Publisher: Molecular Diversity Preservation International (MDPI)
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (2003). A simple and efficient Triton X-100 boiling and chloroform extraction method of RNA isolation from Gram-positive and Gram-negative
  2. (2010). A simple and rapid method of bacterial transformation.
  3. (2010). An improved system for competent cell preparation and high efficiency plasmid transformation using different Escherichia coli strains.
  4. (2008). Aqueous suspension of carbon nanotubes enhances the specificity of long PCR. Biotechniques
  5. (2004). Chestnut bur-shaped aggregates of chrysotile particles enable inoculation of Escherichia coli cells with plasmid DNA.
  6. (2001). Chrysotile asbestos fibers mediate transformation of Escherichia coli by exogenous plasmid
  7. (2007). Discovery and application of the Yoshida effect: Nano-sized acicular materials enable penetration of bacterial cells by sliding friction
  8. (2002). Escherichia coli cells penetrated by chrysotile fibers are transformed to antibiotic resistance by incorporation of exogenous plasmid DNA.
  9. (2002). Mechanism for membrane electroporation irreversibility under high-intensity, ultrashort electrical pulse conditions.
  10. (2005). Multi-walled carbon nanotubes for plasmid delivery into Escherichia coli cells.
  11. (2007). Novel plasmid transformation method mediated by chrysotile, sliding friction, and elastic body exposure.
  12. (2008). Plasmid DNA is released from nanosized acicular material surface by low molecular weight oligonucleotides: exogenous plasmid acquisition mechanism for penetration intermediates based on the Yoshida effect.
  13. (2009). Plasmid uptake by bacteria: a comparison of methods and efficiencies.
  14. (1999). The hazards of chrysotile asbestos: a critical review. Ind. Health