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Receptor Sorting within Endosomal Trafficking Pathway Is Facilitated by Dynamic Actin Filaments

By Emiko Ohashi, Kenji Tanabe, Yuji Henmi, Kumi Mesaki, Yuka Kobayashi and Kohji Takei

Abstract

Early endosomes (EEs) are known to be a sorting station for internalized molecules destined for degradation, recycling, or other intracellular organelles. Segregation is an essential step in such sorting, but the molecular mechanism of this process remains to be elucidated. Here, we show that actin is required for efficient recycling and endosomal maturation by producing a motile force. Perturbation of actin dynamics by drugs induced a few enlarged EEs containing several degradative vacuoles and also interfered with their transporting ability. Actin repolymerization induced by washout of the drug caused the vacuoles to dissociate and individually translocate toward the perinuclear region. We further elucidated that cortactin, an actin-nucleating factor, was required for transporting contents from within EEs. Actin filaments regulated by cortactin may provide a motile force for efficient sorting within early endosomes. These data suggest that actin filaments coordinate with microtubules to mediate segregation in EEs

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3098849
Provided by: PubMed Central

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Citations

  1. (2003). A coat of filamentous actin prevents clustering of late-endosomal vacuoles in vivo.
  2. (2005). A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis.
  3. (2003). A WASp homolog powers actin polymerization-dependent motility of endosomes in vivo.
  4. (2002). Actin dependence of polarized receptor recycling in Madin-Darby canine kidney cell endosomes.
  5. (2010). Actin Dynamics Drive Membrane Reorganization and Scission in Clathrin-Independent Endocytosis.
  6. (2008). Actin dynamics is essential for Myosin-based transport of membrane organelles.
  7. (2001). Actin filaments and myosin I alpha cooperate with microtubules for the movement of lysosomes.
  8. (2007). Actin in membrane trafficking.
  9. (2000). Actindependent propulsion of endosomes and lysosomes by recruitment of N-WASP.
  10. (2009). Annexin A2-dependent polymerization of actin mediates endosome biogenesis.
  11. (2000). Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles.
  12. (2007). Biogenesis and function of multivesicular bodies.
  13. (2005). CART: an Hrs/ actinin-4/BERP/myosin V protein complex required for efficient receptor recycling.
  14. (2000). Characterization of rapid membrane internalization and recycling.
  15. (2006). Cooperation of phosphoinositides and BAR domain proteins in endosomal tubulation.
  16. (2009). Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits.
  17. (2002). Coupling actin dynamics and membrane dynamics during endocytosis.
  18. (2004). Diverse and essential roles of mammalian vacuolar-type proton pump ATPase: toward the physiological understanding of inside acidic compartments.
  19. (2002). Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles.
  20. (2007). Dynein is required for receptor sorting and the morphogenesis of early endosomes.
  21. (2007). Early endosomes associated with dynamic F-actin structures are required for late trafficking of H. pylori VacA toxin.
  22. (2004). Endocytic recycling.
  23. (2002). Endocytosed transferrin receptors recycle via distinct dynamin and phosphatidylinositol 3-kinase-dependent pathways.
  24. (1996). Endocytosis and molecular sorting.
  25. (2009). GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding.
  26. (1983). Groweiss A
  27. (2006). GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission.
  28. (2008). GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission.
  29. (1994). Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin.
  30. (1991). Late endosomes derive from early endosomes by maturation.
  31. (2006). Membrane deformation by protein coats.
  32. (2002). Mosaic organization of the endocytic pathway.
  33. (2003). Multiple roles for Arf6: sorting, structuring, and signaling at the plasma membrane.
  34. (1996). Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and then fuse directly with lysosomes.
  35. (2005). Myosin Ib modulates the morphology and the protein transport within multi-vesicular sorting endosomes.
  36. (2007). Myosin VI and its interacting protein LMTK2 regulate tubule formation and transport to the endocytic recycling compartment.
  37. (2004). Protein kinaseCdelta-calmodulin crosstalk regulates epidermal growth factor receptor exit from early endosomes.
  38. (1992). Receptor-mediated endocytosis in semiintact cells. Meth Enzymol 219: 223–234. Endosomal Trafficking by
  39. (2009). Signaling from endosomes: location makes a difference.
  40. (2007). SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment.
  41. (2007). SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis.
  42. (2001). The actin cytoskeleton is required for the trafficking of the b cell antigen receptor to the late endosomes.
  43. (2009). The BAR domain superfamily: membrane-molding macromolecules.
  44. (2006). The formation of TGN-to-plasma-membrane transport carriers.
  45. (2002). The vacuolar (H+)-ATPases–nature’s most versatile proton pumps.
  46. (2002). Transferrin receptor recycling in the absence of perinuclear recycling endosomes.
  47. (2004). Uncoated endocytic vesicles require the unconventional myosin, Myo6, for rapid transport through actin barriers.
  48. (2006). V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway.