Article thumbnail

Improving evaluation of the distribution and density of immunostained cells in breast cancer using computerized video image analysis

By Brendon J Coventry, Michael J Weightman, John M Skinner and John Bradley


Quantitation of cell density in tissues has proven problematic over the years. The manual microscopic methodology, where an investigator visually samples multiple areas within slides of tissue sections, has long remained the basic ‘standard’ for many studies and for routine histopathologic reporting. Nevertheless, novel techniques that may provide a more standardized approach to quantitation of cells in tissue sections have been made possible by computerized video image analysis methods over recent years. The present study describes a novel, computer-assisted video image analysis method of quantitating immunostained cells within tissue sections, providing continuous graphical data. This technique enables the measurement of both distribution and density of cells within tissue sections. Specifically, the study considered immunoperoxidase-stained tumor infiltrating lymphocytes within breast tumor specimens, using the number of immunostained pixels within tissue sections to determine cellular density and number. Comparison was made between standard manual graded quantitation methods and video image analysis, using the same tissue sections. The study demonstrates that video image techniques and computer analysis can provide continuous data on cell density and number in immunostained tissue sections, which compares favorably with standard visual quantitation methods, and may offer an alternative

Topics: Original Research
Publisher: Dove Medical Press
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. A comparison of the sensitivity of immunoperoxidase staining methods with high-sensitivity fluorescence flow cytometry-antibody quantitation on the cell surface.
  2. A computerized image analysis system for quantitative analysis of cells in histological brain sections.
  3. (1997). Application of computer image analysis in endocrine cell quantification.
  4. Computer-assisted and visual methods of assessing cellular proliferation in tissue sections from non-Hodgkin’s lymphoma.
  5. Density and phenotype of tumour-associated mononuclear cells in colonic carcinoma determined by computer-assisted video image analysis.
  6. Differences between standard and high-sensitivity immunoperoxidase staining methods in tissue sections – comparison of immunoperoxidase staining methods using computerised video image analysis.
  7. (2005). Digital image analysis for the evaluation of the inflammatory infiltrate in psoriasis. Arch Dermatol Res.
  8. (2001). Evaluation of diagnostic efficiency of computerized image analysis based quantitative nuclear parameters in papillary and follicular thyroid tumors using paraffinembedded tissue sections. Pathol Oncol Res.
  9. Immune infiltration in human tumors: a prognostic factor that should not be ignored.
  10. Inflammation and breast cancer. Balancing immune response: crosstalk between adaptive and innate immune cells during breast cancer progression. Breast Cancer Res.
  11. Interpretation and quantification of immunostains.
  12. (1988). Microcomputer video image analysis.
  13. Quantification of immunohistochemistry – issues concerning methods, utility and semiquantitative assessment I.
  14. Quantification of immunohistochemistry – issues concerning methods, utility and semiquantitative assessment II.
  15. Quantification of the cell infiltrate in synovial tissue by digital image analysis.
  16. Quantifying tumour-infiltrating lymphocyte subsets: a practical immunohistochemical method.
  17. Quantitative immunohistochemistry of factor VIII-related antigen in breast carcinoma: a comparison of computer-assisted image analysis with established counting methods.
  18. Reproducibility of immunostaining quantification and description of a new digital image processing procedure for quantitative evaluation of immunohistochemistry in pathology.
  19. The microcomputer and image analysis in diagnostic pathology.
  20. (1990). The use of computer-assisted video image analysis in the enumeration of immuno-stained cells in tissue sections. J Immunol Methods.
  21. (1993). Video image analysis in pathology. Dis Markers.