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Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)

By Eric Abbott, Dawn Hall, Björn Hamberger and Jörg Bohlmann
Topics: Methodology Article
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:3095273
Provided by: PubMed Central

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Citations

  1. (2008). al: A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished fulllength cDNAs for Sitka spruce (Picea sitchensis).
  2. (2007). al: A high quality draft consensus sequence of the genome of a heterozygous grapevine variety. PLoS One
  3. (2009). Amino acid metabolism at the maternal-filial boundary of young barley seeds: a microdissection-based study. Planta
  4. (1977). Anatomy of Seed Plants.
  5. (2008). Application of carborundum abrasion for investigating the leaf epidermis: molecular cloning of Catharanthus roseus 16-hydroxytabersonine-16-O-methyltransferase. Plant J
  6. (2008). Application of laser-assisted microdissection for tissue and cell-specific analysis of RNA, proteins, and metabolites.
  7. (2005). Benfey PN: Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent reporter lines. Nat Methods
  8. (2000). Cambialregion-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene. Plant Physiol
  9. (2007). CJ: Populus: a model system for plant biology. Annu Rev Plant Biol
  10. (2007). Conifer defense against insects: proteome analysis of Sitka spruce (Picea sitchensis) bark induced by mechanical wounding or feeding by white pine weevils (Pissodes strobi). Proteomics
  11. (1991). Defense mechanisms of conifers: Relationship of monoterpene cyclase activity to anatomical Abbott et al.
  12. (2006). Diterpene resin acids in conifers. Phytochemistry
  13. (2008). DR: A systems biology investigation of the MEP/ terpenoid and shikimate/phenylpropanoid pathways points to multiple levels of metabolic control in sweet basil glandular trichomes. Plant J
  14. (2002). E: Application of methyl jasmonate on Picea abies (Pinaceae) stems induces defense-related responses in phloem and xylem.
  15. (2003). E: Inducible anatomical defense responses in Norway spruce stems and their possible role in induced resistance. Tree Physiol
  16. (2007). et al: Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies). Plant Mol Biol
  17. (1998). et al: Gene discovery in the woodforming tissues of poplar: analysis of 5, 692 expressed sequence tags. Proc Natl Acad Sci USA
  18. (2005). et al: Generation, annotation, analysis and database integration of 16,500 white spruce EST clusters.
  19. (2007). et al: Global gene expression analysis of the shoot apical meristem of maize (Zea
  20. (2008). et al: Identification of conserved core xylem gene sets: conifer cDNA microarray development, transcript profiling and computational analyses. New Phytol
  21. (2006). et al: The genome of black cottonwood, Populus trichocarpa
  22. (2005). Evaluation of twodimensional electrophoresis and liquid chromatography–tandem mass spectrometry for tissue-specific protein profiling of laser-microdissected plant samples. Electrophoresis
  23. (2005). Fernald RD: Comprehensive algorithm for quantitative real-time polymerase chain reaction.
  24. (2004). G: A high-resolution transcript profile across the wood-forming meristem of poplar identifies potential regulators of cambial stem cell identity. Plant Cell
  25. (2006). Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defence of conifers against insects and pathogens. New Phytol
  26. (2005). Insect-induced conifer defense. White pine weevil and methyl jasmonate induce traumatic resinosis, de novo formed volatile emissions, and accumulation of terpenoid Abbott et al.
  27. (1992). Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products. Anal Biochem
  28. (2004). JP: A single-vial analytical and quantitative gas chromatography-mass spectrometry assay for terpene synthases. Anal Biochem
  29. (2005). Krekling T: Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytol
  30. (2008). Krekling T: Traumatic resin ducts and polyphenolic parenchyma cells in conifers. Induced Plant Resistance to Herbivory Springer NetherlandsSchaller A
  31. (1996). LA: Laser capture microdissection. Science
  32. (2006). Laser microdissection of plant tissue: what you see is what you get. Annu Rev Plant Biol
  33. (2005). Localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus.
  34. (2008). Luider T: Combining laser capture microdissection and proteomics techniques. Methods Mol Biol
  35. (2008). Mechanism and tissue specificity of nicotine-mediated lung S-adenosylmethionine reduction.
  36. (2002). Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiol
  37. (2007). Microchemical analysis of lasermicrodissected stone cells of Norway spruce by cryogenic nuclear magnetic resonance spectroscopy. Planta
  38. (2006). Pfaffl MW: RNA integrity and the effect on the real-time qRTPCR performance. Mol Aspects Med
  39. (2009). Plant micrometabolomics: The analysis of endogenous metabolites present in a plant cell or tissue.
  40. (2009). Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response. Proteomics
  41. (2008). RC: Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation. Plant Physiol
  42. (1997). Sundberg B: A radial concentration gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen. Plant Physiol
  43. (2009). Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies). Plant J
  44. (2010). Terpenoid biosynthesis and specialized vascular cells of conifer defense.
  45. (2007). The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature
  46. (2004). VR: Induction of anatomically based defense responses in stems of diverse conifers by methyl jasmonate: a phylogenetic perspective. Tree Physiol