Article thumbnail

The centipede genus Eupolybothrus Verhoeff, 1907 (Chilopoda: Lithobiomorpha: Lithobiidae) in North Africa, a cybertaxonomic revision, with a key to all species in the genus and the first use of DNA barcoding for the group

By Pavel Stoev, Nesrine Akkari, Marzio Zapparoli, David Porco, Henrik Enghoff, Gregory D. Edgecombe, Teodor Georgiev and Lyubomir Penev


The centipede genus Eupolybothrus Verhoeff, 1907 in North Africa is revised. A new cavernicolous species, Eupolybothrus kahfi Stoev & Akkari, sp. n., is described from a cave in Jebel Zaghouan, northeast Tunisia. Morphologically, it is most closely related to Eupolybothrus nudicornis (Gervais, 1837) from North Africa and Southwest Europe but can be readily distinguished by the long antennae and leg-pair 15, a conical dorso-median protuberance emerging from the posterior part of prefemur 15, and the shape of the male first genital sternite. Molecular sequence data from the cytochrome c oxidase I gene (mtDNA–5’ COI-barcoding fragment) exhibit 19.19% divergence between Eupolybothrus kahfi and Eupolybothrus nudicornis, an interspecific value comparable to those observed among four other species of Eupolybothrus which, combined with a low intraspecific divergence (0.3–1.14%), supports the morphological diagnosis of Eupolybothrus kahfi as a separate species. This is the first troglomorphic myriapod to be found in Tunisia, and the second troglomorph lithobiomorph centipede known from North Africa. Eupolybothrus nudicornis is redescribed based on abundant material from Tunisia and its post-embryonic development, distribution and habitat preferences recorded. Eupolybothrus cloudsley-thompsoni Turk, 1955, a nominal species based on Tunisian type material, is placed in synonymy with Eupolybothrus nudicornis. To comply with the latest technological developments in publishing of biological information, the paper implements new approaches in cybertaxonomy, such as fine granularity XML tagging validated against the NLM DTD TaxPub for PubMedCentral and dissemination in XML to various aggregators (GBIF, EOL, Wikipedia), vizualisation of all taxa mentioned in the text via the dynamically created Pensoft Taxon Profile (PTP) page, data publishing, georeferencing of all localities via Google Earth, and ZooBank, GenBank and MorphBank registration of datasets. An interactive key to all valid species of Eupolybothrus is made with DELTA software

Topics: Article
Publisher: Pensoft Publishers
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (2005). (1837) Études pour servir á l‘histoire naturelle des Myriapodes. Annales des Sciences naturelles, série 2, 7: 35–60. Hajibabaei
  2. (1841) Arachniden und Myriapoden aus der Regentschaft Algier.
  3. (1849) Exploration scientifi que de l‘Algérie pendant les années 1840, 1841, 1842 publiée par ordre du Gouvernement et avec le concours d‘une Commission Academique.
  4. (1862) Die Myriapodengattung Lithobius.
  5. (1863) Die Myriapoden. Getreu nach der Natur abgebildet und beschrieben,
  6. (1872) Myriapoda Musei Hauniensis. Bidrag til Myriapodernes Morphologi og Systematik.
  7. (1891) Über einige nordafrikanische Chilopoden.
  8. (1896) Una escursione in Tunisia (Symphyla,
  9. (1931b) Sur un Lithobius cavernicole nouveau de l’Algérie.
  10. (2000). (2001a) On centipedes (Chilopoda) of Albania, 2.
  11. (2001b) On the centipedes (Chilopoda) of the Republic of Macedonia. Historia naturalis bulgarica 13:
  12. (2001c) A synopsis of the Bulgarian cave centipedes (Chilopoda).
  13. (2008a) Contribution à l’étude des chilopodes (Chilopoda) des Alpes–Maritimes, incluant une clé d’identifi cation des lithobiomorphes
  14. (2008b) Compléments à la description d’Eupolybothrus (Eupolybothrus) fasciatus (Newport,
  15. (1982). A (1983a) On Sardinian centipedes (Chilopoda). Bollettino di Zoologia 49: 1–16. [for
  16. A (1983b) Note critiche sui Chilopodi della Sardegna. Lavori della Società Italiana di Biogeografi a,
  17. (2002). A Catalogue and Key to the centipedes (Chilopoda) of Bulgaria. Pensoft Publishers, Sofi a-Moscow,
  18. (2006). A catalogue of the centipedes (Chilopoda) of central Apennines (Italy). Bollettino del Museo Civico di Storia Naturale di Verona –
  19. (1970). A redescription of the species of Eupolybothrus Verhoeff s. str. preserved in the British Museum (Natural History) and the Hope Department of Zoology,
  20. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences.
  21. (2004). Adding mitochondrial sequence data (16S rRNA and cytochrome c oxidase subunit I) to the phylogeny of centipedes (Myriapoda: Chilopoda): an analysis of morphology and four molecular loci.
  22. (2001). Algerie. In: Juberthie C, Decu V (Ed.) Encyclopaedia Biospeologica Tome 3, Société de Biospéologie,
  23. (2009). An annotated catalogue of the epigeic and cave centipedes (Chilopoda) of Sardinia. In: Cerretti
  24. (2006). An inexpensive, automation-friendly protocol for recovering high-quality DNA.
  25. (1973). Beiträge zur Kenntnis bulgarischer Chilopoden. Teil V. Das Genus Polybothrus.
  26. (1901). Beiträge zur Kenntnis paläarktischer Myriopoden. XVI Aufsatz. Zur vergleichenden Morphologie,
  27. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Software available at: bioedit.html Hausmann
  28. (2007). BOLD: Th e Barcode of Life Data System ( Molecular Ecology Notes: 1–10. doi:10.1111/j.1471-8286.2006.01678.x. Saitou
  29. (2006). CHILOBASE. A web resource for Chilopoda taxonomy. http:// Minelli A, Iovane E
  30. (1955). Concerning the genotypes of Bothropolys, Polybothrus and Eupolybothnis (Chilopoda: Lithobiomorpha: Lithobiidae). Entomological News 66 (4): 107–110.Pavel Stoev et al.
  31. (1995). corologia e note ecologiche su Eupolybothrus (Eupolybothrus) litoralis (Chilopoda,
  32. (1967). Description d’une espèce nouvelle cavernicole de Lithobius (Lithobiomorpha, Chilopoda) de l’Algérie.
  33. (1996). Développement post-embryonnaire et cycle biologique de Eupolybothrus elongatus (Newport) dans l’est algérien. In:
  34. (2003). Etude de parametres biologiques chez Eupolybothrus nudicornis (Myriapode Chilopode) en vue de leur utilisation pour l’evaluation de la toxicite de composes chimiques utilises en agriculture. Bulletin de la Société zoologique de France
  35. (1994). Faunistica ed ecologia dei chilopodi dei Monti Ausoni e dei Monti Aurunci (Lazio) (Chilopoda). Bollettino dell’Associazione Romana di
  36. (1952). Franz in Spanien
  37. (2006). Geological Atlas of Africa,
  38. (1992). Hurezeanu A
  39. (1985). I Chilopodi della regione ligure con particolare riguardo al popolamento delle Alpi Liguri. Lavori della Societa Italiana di Biogeografi a,
  40. (1921). Liste des Myriapodes signalés dans le nord de l’Afrique.
  41. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
  42. (1956). Miriapodi cavernicoli del Marocco, della Sardegna e del Piemonte. Atti della Società italiana di Scienze naturali, Museo Civico di storia naturale 95:
  43. (1968). Myriapoda-Chilopoda aus der Sammlung des Musée Royal de l’Afrique Centrale.
  44. (1931). Myriapodes recueillis par M. le Dr H. Gauthier en Algérie. Bulletin de la Société d’Histoire Naturelle de l’Afrique du
  45. (1991). Nota su alcune specie di Chilopodi della regione palestinese.
  46. (1908). Note sur les Myriapodes recueillis par M. Henri Gadeau de Kerville en Khroumirie et description de deux espèces et d’une variété nouvelles provenant de cette région de la Tunisie. In: Gadeau de Kerville H (Ed.). Voyage zoologique en Khroumirie (Tunisie),
  47. (1979). Nouveautes sur la faune des Chilopodes de Yougoslavie. Biološki vestnik,
  48. (1940). O nových balkánských jeskennich Chilopodech ve sběrech
  49. (1980). On Lithobiidae from Sardinia (Chilopoda: Lithobiomorpha). Bulletin Zoölogisch Museum, Universiteit van
  50. (1967). On two italian Lithobius species described by Silvestri with taxonomic notes on the genus Eupolybothrus Verhoeff (Chilopoda Lithobiidae).
  51. (1984). Osservazioni sui Litobiomorfi cavernicoli del Maghreb (Chilopoda, Lithobiomorpha).
  52. (2010). Paris, 527 pp.Pavel Stoev et al.
  53. (2008). Postlarval development of Eupolybothrus transsylvanicus (Latzel) (Chilopoda: Lithobiomorpha) from Serbia. In: Makarov S, Dimitrijević R (Eds) Advances in Arachnology and Developmental Biology. Papers dedicated to Prof. Dr. Božidar Ćurčić.
  54. (1925). Races nouvelles de Schizophyllum algériens (Myriapodes-Diplopodes). Bulletin de la Société de l’Histoire Naturelle de l’Afrique du
  55. (1993). Rifl essioni di gruppo sui corotipi fondamentali della fauna W-paleartica ed in particolare italiana. Biogeographia, Lavori della Società Italiana di Biogeografi a, Nuova Serie 16: 159–179.Pavel Stoev et al.
  56. (2010). Semantic tagging of and semantic enhancements to systematics papers: ZooKeys working examples.
  57. (1956). Studies in diurnal rhythms, VI. Bioclimatic observations in Tunisia and their signifi cance in relation to the physiology of the fauna especially woodlice, centipedes, scorpions, beetles.
  58. (1932). Tableaux de détermination des Chilopodes signalés en Afrique du Nord.
  59. (1981). Taxonomical studies on the post-embryotic development in Swedish Lithobiomorpha (Chilopoda). Entomologica scandinavica,
  60. (1925). Th e Ethopolidae of America north of Mexico.
  61. (1963). Th e generic and subgeneric names of the European Lithobidae generally referred to Polybothrus (Latzel,
  62. (1983). Th e identity of the European and Mediterranean species of Lithobiidae (Chilopoda) described by K. W. Verhoeff and now represented by material preserved in the British Museum (Natural History).
  63. (1958). Th e life-history of Bothropolys asperatus
  64. (1955). Th e Myriapoda of Dr. Cloudsley-Th ompson’s expedition to the Tunisian desert.
  65. (2003). Th e present knowledge on the European fauna of Lithobiomorpha (Chilopoda).
  66. (2006). Type specimens of the Lithobiomorpha (Chilpoda)
  67. (1936). Über einige Myriapoden und einen Isopoden aus mediterranen Höhlen. Mitteilungen von Höhlen-
  68. (1998). Una nuova specie di Eupolybothrus della fauna di Grecia (Chilopoda,
  69. (1966). Une classifi cation biogéographique des climats. Recherches et Travaux des Laboratoires de Géologie,