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Detection of Head-to-Tail DNA Sequences of Human Bocavirus in Clinical Samples

By Jessica Lüsebrink, Verena Schildgen, Ramona Liza Tillmann, Felix Wittleben, Anne Böhmer, Andreas Müller and Oliver Schildgen


Parvoviruses are single stranded DNA viruses that replicate in a so called “rolling-hairpin” mechanism, a variant of the rolling circle replication known for bacteriophages like ϕX174. The replication intermediates of parvoviruses thus are concatemers of head-to-head or tail-to-tail structure. Surprisingly, in case of the novel human bocavirus, neither head-to-head nor tail-to-tail DNA sequences were detected in clinical isolates; in contrast head-to-tail DNA sequences were identified by PCR and sequencing. Thereby, the head-to-tail sequences were linked by a novel sequence of 54 bp of which 20 bp also occur as conserved structures of the palindromic ends of parvovirus MVC which in turn is a close relative to human bocavirus

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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