Location of Repository

Differences in Spatio-Temporal Behavior of Zebrafish in the Open Tank Paradigm after a Short-Period Confinement into Dark and Bright Environments

By Denis B. Rosemberg, Eduardo P. Rico, Ben Hur M. Mussulini, Ângelo L. Piato, Maria E. Calcagnotto, Carla D. Bonan, Renato D. Dias, Rachel E. Blaser, Diogo O. Souza and Diogo L. de Oliveira

Abstract

The open tank paradigm, also known as novel tank diving test, is a protocol used to evaluate the zebrafish behavior. Several characteristics have been described for this species, including scototaxis, which is the natural preference for dark environments in detriment of bright ones. However, there is no evidence regarding the influence of “natural stimuli” in zebrafish subjected to novelty-based paradigms. In this report, we evaluated the spatio-temporal exploratory activity of the short-fin zebrafish phenotype in the open tank after a short-period confinement into dark/bright environments. A total of 44 animals were individually confined during a 10-min single session into one of three environments: black-painted, white-painted, and transparent cylinders (dark, bright, and transparent groups). Fish were further subjected to the novel tank test and their exploratory profile was recorded during a 15-min trial. The results demonstrated that zebrafish increased their vertical exploratory activity during the first 6-min, where the bright group spent more time and travelled a higher distance in the top area. Interestingly, all behavioral parameters measured for the dark group were similar to the transparent one. These data were confirmed by automated analysis of track and occupancy plots and also demonstrated that zebrafish display a classical homebase formation in the bottom area of the tank. A detailed spatio-temporal study of zebrafish exploratory behavior and the construction of representative ethograms showed that the experimental groups presented significant differences in the first 3-min vs. last 3-min of test. Although the main factors involved in these behavioral responses still remain ambiguous and require further investigation, the current report describes an alternative methodological approach for assessing the zebrafish behavior after a forced exposure to different environments. Additionally, the analysis of ethologically-relevant patterns across time could be a potential phenotyping tool to evaluate the zebrafish exploratory profile in the open tank task

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3085514
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Preview

    Citations

    1. (2006). A behavioral homeostasis theory of habituation and sensitization: II. Further developments and predictions.
    2. (2008). A comparative analysis of the preference for dark environments in five teleosts.
    3. (1990). A computational theory of the hippocampal cognitive map.
    4. (2008). Altered responses to novelty and drug reinforcement in adult rats treated neonatally with domoic acid.
    5. (2010). Analyzing habituation responses to novelty in zebrafish (Danio rerio).
    6. (2010). Antipsychotic drugs prevent the motor hyperactivity induced by psychotomimetic MK-801 in zebrafish (Danio rerio).
    7. (2008). Anxiogenic effects of cocaine withdrawal in zebrafish.
    8. (2007). Anxiolytic effects of nicotine in zebrafish.
    9. (2010). Behavioral measures of anxiety in zebrafish (Danio rerio).
    10. (2007). Behavioral neuroscience, exploration, and K.C. Montgomery’s legacy.
    11. (2006). Behavioral phenotyping in zebrafish: comparison of three behavioral quantification methods.
    12. (2009). Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety.
    13. (2010). Characterization of behavioral and endocrine effects of LSD on zebrafish.
    14. (2006). Cognitive aging in zebrafish.
    15. (2005). Computational characterization of behavioral response of medaka (Oryzias latipes) treated with diazinon.
    16. (2008). Domain interplay concept in animal models of neuropsychiatric disorders: a new strategy for high-throughput neurophenotyping research.
    17. (2006). Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio).
    18. (1985). Exploratory behavior models of anxiety in mice.
    19. (2006). Habituation in rodents: a review of behavior, neurobiology, and genetics.
    20. (1966). Habituation: a model phenomenon for the study of neuronal substrates of behavior.
    21. Herculano AM (2011) Pharmacological analysis of zebrafish (Danio rerio) scototaxis. Prog NeuroPsychopharmacol Biol Psychiatry doi:10.1016/j.pnpbp.2011.01.006.
    22. (2010). High-throughput behavioral screens: the first step towards finding genes involved in vertebrate brain function using zebrafish.
    23. (1989). Home base behavior of rats (Rattus norvegicus) exploring a novel environment.
    24. (2010). Homebase behavior of zebrafish in novelty-based paradigms.
    25. (2011). Identification of a brain center whose activity discriminates a choice behavior in zebrafish.
    26. (2010). Measuring anxiety in zebrafish: a critical review.
    27. (2010). Morato S
    28. (2005). Movement behaviour of Medaka (Oryzias latipes) in response to sublethal treatments of diazinon and cholinesterase activity in semi-natural conditions.
    29. (1999). Natural preference of zebrafish (Danio rerio) for a dark environment.
    30. (2003). Open-field behavior withstands drastic changes in arena size.
    31. (1975). Open-field behaviour of oestrous and dioestrous rats: evidence against an ‘emotionality’ interpretation.
    32. (2010). Parametric analyses of anxiety in zebrafish scototaxis.
    33. (2001). Rats and mice share common ethologically relevant parameters of exploratory behavior.
    34. (2000). Rosenthal A
    35. (2005). Sharing of the home base: a social test in rats.
    36. (1993). Structure of the rat’s behaviour in the hot plate test.
    37. (2009). The ontogeny of exploratory behavior in male and female adolescent rats (Rattus norvegicus).
    38. (1976). The Open-Field Test: a critical review.
    39. (2008). Thigmotactic responses in an open-field.
    40. (2011). Threedimensional neurophenotyping of adult zebrafish behavior.
    41. (2000). Topographical evaluation of behavioural phenotype in a line of mice with targeted gene deletion of the D2 dopamine receptor.
    42. (2009). Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish.
    43. (2005). Variations in the behaviors to novel objects among five inbred strains of mice.
    44. (2003). Zebra fish: an uncharted behavior genetic model.
    45. (2010). Zebrafish antipredatory responses: a future for translational research?
    46. (2010). Zebrafish behavior in novel environments: Effects of acute exposure to anxiolytic compounds and choice of Danio rerio line.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.