Article thumbnail

Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts

By Toshiyuki Hagiwara, Takashi Ushimaru, Kei-ichi Tainaka, Hironori Kurachi and Jin Yoshimura

Abstract

Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known to reach at 20–30 stages on an isolated agar medium. However, their stage dynamics in a liquid culture is virtually unknown. We investigate the population dynamics by counting scars in each cell. Here one cell division produces one new cell and one bud scar. This simple rule leads to a conservation law: “The total number of bud scars is equal to the total number of cells.” We find a large discrepancy: extremely fewer cells with over 5 scars than expected. Almost all cells with 6 or more scars disappear within a short period of time in the late log phase (corresponds to the inflection point). This discrepancy is confirmed directly by the microscopic observations of broken cells. This finding implies apoptosis in older cells (6 scars or more)

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3083425
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2009). Aging defined by a chronologic-replicative protein network in Saccharomyces cerevisiae: an interactome analysis.
  2. (2004). CDK Activity Antagonized Whi5, an inhibitor of G1/S Transcription in yeast.
  3. (2011). Cell size and growth rate are major determinants of replicative life span.
  4. (2008). Chronological aging-induced apoptosis in yeast.
  5. (2001). Chronological lifespan of stationary phase yeast cells; a model for investigating the factors that might influence the ageing of postmitotic tissues in higher organisms.
  6. (1977). Coordination of growth and cell division in the yeast Saccharomyces cerevisiae.
  7. (1998). Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.
  8. (2009). Dividing cellular asymmetry: asymmetric cell division and its implications for stem cells and cancer.
  9. (1999). Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast.
  10. (1986). Genealogy of principal strains of the yeast genetic stock center.
  11. (2005). Genes determining yeast replicative life span in a long lived genetic background.
  12. (1974). Genetic control of the cell division cycle in yeast; a model.
  13. (1959). Life span of individual yeast cells.
  14. (2003). Longevity regulation in Saccharomyces cerevisiae: linking metabolism, genome stability, and heterochromatin.
  15. (2009). Measuing replicative life span in the budding yeast.
  16. (2008). Modeling stem cell asymmetry in yeast.
  17. (1982). On the oscillatory transient stage structure of yeastpopulation.
  18. (2008). Replicative aging in yeast: the means to the end.
  19. (1974). Saccharomyces cerevisiae cell cycle.
  20. (1985). Stage dependent density effect on yeast population.
  21. (2006). Stage-dependent density effect in the cell cycle of budding yeast.
  22. (1982). Stationary stage structure of yeast population with stage dependent generation time.
  23. (1977). Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division.