Skip to main content
Article thumbnail
Location of Repository

A transferable heterogeneous two-hybrid system in Escherichia coli based on polyhydroxyalkanoates synthesis regulatory protein PhaR

By Zhi-Hui Wang, Ping Ma, Jiong Chen, Jing Zhang, Chong-Bo Chen and Guo-Qiang Chen
Topics: Research
Publisher: BioMed Central
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (2007). A bacterial two-hybrid system based on the twin-arginine transporter pathway of E. coli. Protein Sci
    2. (2002). A repressor protein, PhaR, regulates polyhydroxyalkanoate (PHA) synthesis via its direct interaction with PHA.
    3. (1999). A: A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol
    4. (1995). Analysis of ATF2 gene expression during early Xenopus laevis development. Gene
    5. (2007). Autoregulator protein PhaR for biosynthesis of polyhydroxybutyrate [P (3HB)] possibly has two separate domains that bind to the target DNA and P (3HB): functional mapping of amino acid residues responsible for DNA binding.
    6. (1990). Dawes E: Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev
    7. (1995). F: Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus.
    8. (1995). Finding prospective partners in the library: the two-hybrid system and phage display find a match. Trends Biochem Sci
    9. (2008). GQ: A novel selfcleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles. Lab Chip
    10. (1999). Huisman G: Metabolic engineering of poly (3-hydroxyalkanoates): from DNA to plastic. Microbiol Mol Biol Rev
    11. (2003). Kerppola TK: Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol
    12. (1998). Ladant D: A bacterial two-hybrid system based on a reconstituted signal transduction pathway.
    13. (2001). Molecular characterization of a regulatory protein (PhaR) involved in PHA biosynthesis. RIKEN Rev
    14. (1997). Molecular mass of poly [(R)-3-hydroxybutyric acid] produced in a recombinant Escherichia coli. Appl Microbiol Biotechnol
    15. (2005). Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation. Nucleic Acids Res
    16. (1989). Preferential heterodimer formation by isolated leucine zippers from fos and jun. Science
    17. (1993). Selective DNA bending by a variety of bZIP proteins.
    18. (1983). SG: Reliability of the Fugl-Meyer assessment of sensorimotor recovery following cerebrovascular accident. Phys Ther
    19. (1996). Srienc F: Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate.
    20. (2008). Stagljar I: Two-hybrid technologies in proteomics research. Curr Opin Biotechnol
    21. (1991). The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
    22. (2006). Visualization of molecular interactions by fluorescence complementation. Nat Rev Mol Cell Biol

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.