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aCGH.Spline—an R package for aCGH dye bias normalization

By Tomas W. Fitzgerald, Lee D. Larcombe, Solena Le Scouarnec, Stephen Clayton, Diana Rajan, Nigel P. Carter and Richard Redon


Motivation: The careful normalization of array-based comparative genomic hybridization (aCGH) data is of critical importance for the accurate detection of copy number changes. The difference in labelling affinity between the two fluorophores used in aCGH—usually Cy5 and Cy3—can be observed as a bias within the intensity distributions. If left unchecked, this bias is likely to skew data interpretation during downstream analysis and lead to an increased number of false discoveries

Topics: Original Papers
Publisher: Oxford University Press
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Provided by: PubMed Central
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