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Prevalence of Listeria monocytogenes in Raw Meats Marketed in Bangkok and Characterization of the Isolates by Phenotypic and Molecular Methods

By Nitaya Indrawattana, Tanaporn Nibaddhasobon, Nitat Sookrung, Manas Chongsa-nguan, Anchalee Tungtrongchitr, Sou-ichi Makino, Witawat Tungyong and Wanpen Chaicumpa

Abstract

Listeria monocytogenes causes listeriosis characterized by septicaemia, encephalitis, and abortion or stillbirth. Regular monitoring of its prevalence in food and characterization of its phenotypes and genotypes are necessary for disease surveillance and tracing the epidemic outbreaks. In this study, the prevalence of L. monocytogenes in raw meats marketed in Bangkok was 15.4%. The bacteria isolated from meat were serotyped and genotyped using enterobacterial repetitive intergenic consensus–polymerase chain reaction (ERIC-PCR). Their virulence-associated genes, antimicrobial susceptibility, and ability to invade intestinal epithelial cells were studied. All 22 L. monocytogenes strains isolated from 104 raw meat samples carried virulence-associated genes, such as actA, flaA, hlyA, iap, inlA, inlB, and prfA. These were serotype 4b, suggesting their pathogenic and epidemic potential. These isolates could be classified into six ERIC-PCR groups: A-F. The majority (59.1%) of the isolates belonged to Group A, and three isolates were Group D which was closely related to the Group A. Two isolates each were Group C and E, and one isolate each was group B and F. Although the isolates belonged to the same serotype and genotype and were all equipped with the virulence-associated genes, they showed a different cell invasion capability and antibiotic susceptibility. All the isolates were susceptible to ampicillin, amikacin, chloramphenicol, gentamicin, imipenem, penicillin G, sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate showed only intermediate susceptibility to tetracycline. The data provide the first molecular insight into the L. monocytogenes isolates in Thailand and elucidate a potential risk of people contracting listeriosis

Topics: Original Papers
Publisher: International Centre for Diarrhoeal Disease Research, Bangladesh
OAI identifier: oai:pubmedcentral.nih.gov:3075055
Provided by: PubMed Central

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Citations

  1. Bacterial penetration across the blood-brain barrier during the development of neonatal meningitis.
  2. Behavior of Listeria monocytogenes at 4 °C and 22 °C in whey and skim milk containing 6 or 12% sodium chloride.
  3. Biosensors and bio-based methods for the separation and detection of foodborne patho-Indrawattana N et al.
  4. Brain abcess due to Listeria monocytogenes: first case report in Thailand.
  5. Caring for a patient with Listeria endocarditis: use of antibiotic desensitization.
  6. Characterization of Listeria monocytogenes isolated from retail foods.
  7. (1999). Differential expression of Listeria monocytogenes virulence genes in mammalian host cells.
  8. DNA fingerprinting by ERIC-PCR for comparing Listeria spp. strains isolated from different sources in San Luis,
  9. Effect of flagella on initial attachment of Listeria monocytogenes to stainless steel.
  10. (1998). Effects of pH or aw stress on growth of Listeria monocytogenes.
  11. Epidemic listeriosis associated with Mexican-style cheese.
  12. (1991). Epidemiology of human listeriosis.
  13. Flagellar motility is critical for Listeria monocytogenes biofilm formation.
  14. Foodborne microbial pathogens: mechanisms and pathogenesis.
  15. (2009). genetic diversity and antimicrobial susceptibility of Listeria monocytogenes isolated from open-air food markets in Greece. Food Control
  16. Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes.
  17. Genetic markers unique to Listeria monocytogenes 4b differentiate epidemic line II (hot dog outbreak strains) from other lineages.
  18. Genital listeriosis as a cause of repeated abortion.
  19. Genotype characterization of Listeria monocytogenes isolated from foodstuffs and farm animals in Poland.
  20. (2011). Genotypic characterization of Listeria Indrawattana N et al. Listeria monocytogenes isolated in
  21. (1978). In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun
  22. (1990). Incidence and characterization of Listeria monocytogenes in foods available in Taiwan.
  23. (2009). Incidence of Listeria monocytogenes in beef, pork, raw-dried and raw-smoked sausages in Bulgaria. Food Control
  24. Incidence of Listeria monocytogenes in raw seafood products in Japanese retail stores.
  25. Infection and stillbirth.
  26. Influence of pH, water activity and acetic acid concentration on Listeria monocytogenes at 7 °C: data collection for the development of a growth/no growth model.
  27. Interaction of Listeria monocytogenes with human brain microvascular endothelial cells: InlB-dependent invasion, long-term intracellular growth, and spread from macrophages to endothelial cells. Infect Immun1998;66:5260-7.
  28. Interaction of Listeria monocytogenes with the intestinal epithelium.
  29. Involvement of closely related strains of a new clonal group of Listeria monocytogenes in the 1989-99 and 2002 multistate outbreaks of foodborne listeriosis in the United States. Foodborne Pathog Dis
  30. (2006). Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Vet Microbiol
  31. (1995). Listeria meningitis: report of two cases in Thailand.
  32. (1999). Listeria monocytogenes exploits normal host cell processes to spread from cell to cell.
  33. (2006). Listeria monocytogenes invades the epithelial junctions at sites of cell extrusion.
  34. (2003). Listeria monocytogenes meningitis and rhombencephalitis. Pathol Case Rev
  35. (1991). Listeria monocytogenes, a foodborne pathogen. Microbiol Rev
  36. Listeria pathogenesis and molecular virulence determinants.
  37. Listeria: a foodborne pathogen that knows how to survive.
  38. Listeriosis in pregnancy: diagnosis, treatment, and prevention.
  39. Listeriosis: a primer.
  40. Loss of flagellum-based motility by Listeria monocytogenes results in formation of hyperbiofilms.
  41. Mechanisms of microbial traversal of the blood–brain barrier.
  42. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation.
  43. Modeling the growth kinetics of Listeria as a function of temperature, pH and organic acid concentration.
  44. (1994). Molecular and genetic determinants of the Listeria monocytogenes infectious process. Curr Top Microbiol Immunol
  45. Nationwide outbreak of listeriosis due to contaminated meat.
  46. Neonatal meningitis due to Listeria monocytogenes after 3 weeks of maternal treatment during pregnancy.
  47. (2007). New consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of frequently isolated or fastidious bacteria. Clin Infect Dis
  48. Occurrence of Listeria monocytogenes in fresh and processed foods in
  49. (1996). Overview of listeriosis.
  50. Prevalence of foodborne pathogens in open markets and supermarkets in Thailand. Food Control
  51. (2000). Prevalence of Listeria in Chinese food products from 13 provinces between
  52. Requirement of the Listeria monocytogenes broad-range phospholipase PC-PLC during infection of human epithelial cells.
  53. (1996). Ribotype diversity of Listeria monocytogenes strains associated with outbreaks of listeriosis in ruminants.
  54. Role of hemolysin for the intracellular growth of Listeria monocytogenes.
  55. Role of listeriolysin O in cell-to-cell spread of Listeria monocytogenes.
  56. Suptyping Listeria monocytogenes through the combined analyses of genotype and expression of the hlyA virulence determinant.
  57. Survival strategies of infectious biofilms.
  58. The ClpP serine protease is essential for the intracellular parasitism and virulence of Listeria monocytogenes.
  59. The epidemiology of human listeriosis.
  60. The use of host cell machinery in the pathogenesis of Listeria monocytogenes.
  61. Treatment of listeriosis.
  62. (1999). Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.
  63. Why are bacteria refractory to antimicrobials?