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Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn5Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle▿ †

By Sabine E. Eckert, Francis Dziva, Roy R. Chaudhuri, Gemma C. Langridge, Daniel J. Turner, Derek J. Pickard, Duncan J. Maskell, Nicholas R. Thomson and Mark P. Stevens

Abstract

Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use

Topics: Molecular Biology of Pathogens
Publisher: American Society for Microbiology (ASM)
OAI identifier: oai:pubmedcentral.nih.gov:3067669
Provided by: PubMed Central
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