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A label-free biosensor based on silver nanoparticles array for clinical detection of serum p53 in head and neck squamous cell carcinoma

By Wei Zhou, Yingyi Ma, Huan Yang, Yi Ding and Xiangang Luo

Abstract

By using triangular silver (Ag) nanoparticle array, a localized surface plasmon resonance (LSPR) nanosensor was fabricated and shown to sense serum p53 protein in vitro, which is involved in head and neck squamous cell carcinoma (HNSCC). The nanosensor consists of a triangular Ag nanoparticles array with single particle dimension of 120 nm in-plane width and 45 nm out-of-plane height. When examined using LSPR nanobiosensor, the results indicated significant difference in LSPR shifts (Δλmax) between HNSCC patient and control. Although there is need for precise quantification and large-scale prospective, this report shows that the LSPR nanobiosensor provides a promising platform with attractive advantages for serological diagnosis or molecular diagnosis in tumor, such as HNSCC. This is the first clinical application of the LSPR nanosensor in HNSCC

Topics: Case Report
Publisher: Dove Medical Press
OAI identifier: oai:pubmedcentral.nih.gov:3065794
Provided by: PubMed Central

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Citations

  1. (2004). A localized surface plasmon resonance biosensor: first steps toward an assay for Alzheimer’s disease. Nano Lett.
  2. Clinical significance of serum p53 antigen in patients with pancreatic carcinoma.
  3. (2008). Colloidal-based localized surface plasmon resonance (LSPR) biosensor for the quantitative determination of stanozolol. Anal Bioanal Chem.
  4. Detection of serum p53 protein in lung cancer patients.
  5. Electromagnetic fields around silver nanoparticles and dimers.
  6. High frequency of p53 gene alterations associated with protein overexpression in human squamous cell carcinoma of larynx.
  7. (1995). Levels of p53 antigen in the plasma of patients with adenomas and carcinomas of the colon. Cancer Lett.
  8. Localized surface plasmon resonance biosensors.
  9. (2009). Localized surface plasmon resonance-based hybrid Au–Ag nanoparticles for detection of Staphylococcus aureus enterotoxin B. Opt Mater.
  10. (1994). P53 tumor suppressor oncogene expression in squamous cell carcinoma of hypopharynx.
  11. (2001). Prognostic significance of serum p53 protein and p53 antibody in patients with surgical treatment for head and neck squamous cell carcinoma. Head Neck.
  12. (1994). Tumor angiogenesis, the p53 antigen, and cervical metastasis in squamous cell carcinoma of the tongue. Otolaryngol Head Neck Surg.