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The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling

By Juliane Schmidt, Mathias Müsken, Tanja Becker, Zofia Magnowska, Daniela Bertinetti, Stefan Möller, Bastian Zimmermann, Friedrich W. Herberg, Lothar Jänsch and Susanne Häussler


The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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  1. (1998). A
  2. (2005). A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels.
  3. (1978). A protein methylesterase involved in bacterial sensing.
  4. (1988). Acetyladenylate plays a role in controlling the direction of flagellar rotation.
  5. (2004). Aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces.
  6. (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants.
  7. (2010). Antibiotic resistance of bacterial biofilms.
  8. (2002). Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants.
  9. (1999). Bacterial biofilms: a common cause of persistent infections.
  10. (1997). Bacterial chemotaxis: Rhodobacter sphaeroides and Sinorhizobium meliloti–variations on a theme? Microbiology 143(Pt 12):
  11. (2006). BdlA, a chemotaxis regulator essential for biofilm dispersion in Pseudomonas aeruginosa.
  12. (2003). Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants.
  13. (2004). Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.
  14. (1994). Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.
  15. (2007). Characterization of the Thermotoga maritima chemotaxis methylation system that lacks pentapeptide-dependent methyltransferase CheR:MCP tethering.
  16. (2007). Chemosensory pathways, motility and development in Myxococcus xanthus.
  17. (1998). Chemotactic adaptation is altered by changes in the carboxy-terminal sequence conserved among the major methyl-accepting chemoreceptors.
  18. (1966). Chemotaxis in bacteria.
  19. (1998). Chemotaxis receptor recognition by protein methyltransferase CheR.
  20. (2009). Chemotaxis-like regulatory systems: unique roles in diverse bacteria.
  21. (1999). Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.
  22. (2002). Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response.
  23. (2000). Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.
  24. (2003). Comprehensive transposon mutant library of Pseudomonas aeruginosa.
  25. (1994). Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication
  26. (2001). Cystic fibrosis pathogenesis and the role of biofilms in persistent infection.
  27. (2000). Development and dynamics of Pseudomonas sp.
  28. (2004). Discrimination between different methylation states of chemotaxis receptor Tar by receptor methyltransferase CheR.
  29. (2004). Diversity in chemotaxis mechanisms among the bacteria and archaea.
  30. (2002). Dual recognition of the bacterial chemoreceptor by chemotaxis-specific domains of the CheR methyltransferase.
  31. (2009). Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome.
  32. (1998). Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development.
  33. (2007). Flagellar motility is critical for Listeria monocytogenes biofilm formation.
  34. (1998). Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili.
  35. (2004). Genetic evidence that the Vibrio cholerae monolayer is a distinct stage in biofilm development.
  36. (1997). Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa.
  37. (2010). Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains.
  38. Ha ¨se CC (2004) Chemotaxis in Vibrio cholerae.
  39. (2010). Ha ¨ussler S
  40. (1995). How bacteria sense and swim.
  41. (1977). Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system.
  42. (1998). Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis.
  43. (1995). Isolation and characterization of chemotaxis mutants and genes of Pseudomonas aeruginosa.
  44. (1995). Microbial biofilms.
  45. (1995). Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa.
  46. (2002). Molecular information processing: lessons from bacterial chemotaxis.
  47. (2007). Motility and chemotaxis in Agrobacterium tumefaciens surface attachment and biofilm formation.
  48. (2007). Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa.
  49. (1989). Phosphorylation of an N-terminal regulatory domain activates the CheB methylesterase in bacterial chemotaxis.
  50. (2007). PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.
  51. (2008). Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem.
  52. (2005). Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating P. aeruginosa Chemotaxis and Biofilm Formation
  53. (1983). Pu ¨hler A
  54. (1987). Purification and characterization of the S-adenosylmethionine:glutamyl methyltransferase that modifies membrane chemoreceptor proteins in bacteria.
  55. (1989). Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants.
  56. (2008). Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms.
  57. (1991). Sites of deamidation and methylation in Tsr, a bacterial chemotaxis sensory transducer.
  58. (2009). Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR.
  59. (1991). The kinetic mechanism of S-adenosyl-Lmethionine: glutamylmethyltransferase from Salmonella typhimurium.
  60. (2009). The molecular basis of excitation and adaptation during chemotactic sensory transduction in bacteria.
  61. (1996). The receptor binding site for the methyltransferase of bacterial chemotaxis is distinct from the sites of methylation.
  62. (1994). The serine receptor of bacterial chemotaxis exhibits half-site saturation for serine binding.
  63. (2006). Two different Pseudomonas aeruginosa chemosensory signal transduction complexes localize to cell poles and form and remould in stationary phase.