Article thumbnail

Vero cell co-culture counteracts the detrimental effects of hydrosalpinx fluid on the development of mouse embryos in vitro.

By Yong Bong Kim, Sung Ho Ahn, Doo Young Chang, Kyung Nam Chung and Jae Whoan Koh

Abstract

Recent studies have suggested that the hydrosalpinx has a negative effect on pregnancy outcome, with markedly diminished implantation and increased early pregnancy loss. Fluid from the hydrosalpinx may leak into and accumulate in the uterine cavity. It is not clear, however if this creates a hostile local environment in the uterus for embryo implantation or exerts a direct embryotoxic effect. This study was conducted to investigate the detrimental effects of hydrosalpinx fluid (HSF) on the development of mouse embryos in vitro and to demonstrate whether Vero cells overcome these adverse effects. HSF was collected from three women with bilateral hydrosalpinx at the time of laparoscopic surgery. Collected fluid was centrifuged and the supernatant was frozen at -20 degrees C. For co-culture, Vero cells were commercially obtained in a frozen state and cultured using Ham's F10 medium. Single-cell mouse embryos (B6CBAF1) were cultured for 5 days in 0, 0.4, 0.8, and 1.2% of HSF in media with and without Vero cells and examined daily to record the number of embryos reaching expanded blastocyst and hatching stage. Co-culture of mouse embryos with Vero cells at 0.8% HSF concentration significantly enhanced embryo development, but not at 1.2% hydrosalpinx fluid concentration. These results suggest that HSF is highly embryotoxic and Vero cells are likely to overcome these detrimental effects to some degree

Topics: Research Article
Publisher: Korean Academy of Medical Sciences
OAI identifier: oai:pubmedcentral.nih.gov:3054843
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (1990). Co-culture of 1-cell mouse embryos on different cell supports. Hum Reprod
  2. (1992). Cytokines: involvement in reproduction Fertil Steril
  3. (1993). Development of human embryos to the hatched blastocyst stage in the presence or absense of a monolayer of Vero cells. Hum Reprod
  4. (1989). Effect of oviductal environment on embryonic development.
  5. Embryotoxicity of hydrosalpinx fluid. Presented at the 43rd annual meeting of the Society of Gynecologic Investigators
  6. (1991). Fluid accumulation of the uterine cavity before embryo transfer: a possible hindrance for implantation. J In Vitro Fert Embryo Transfer
  7. (1996). Gynecol Invest
  8. (1969). Human hydrosalpinx: histologic study and chemical composition of fluid.
  9. (1995). Hydrosalpinges in in-vitro fertilization: an unfavorable prognostic feature. Hum Reprod
  10. (1996). Hydrosalpinx fluid has embryotoxic effects on murine embryogenesis: a case for prophylactic salpingectomy. Fertil Steril
  11. (1994). Hydrosalpinx reduces in-vitro fertilization/embryo transfer pregnancy rates. Hum Reprod
  12. (1992). Improved development of human embryo in vitro by a human oviductal cell co-culture system. Hum Reprod
  13. (1990). Improvement of human early embryo development in vitro by co-culture on monolayers of Vero cells. Biol Reprod
  14. (1989). In-vitro cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells. Reprod Fertil Dev
  15. (1989). In-vitro development and implantation of human embryos following culture on fetal bovine uterine fibroblast cells. Hum Reprod
  16. (1994). Reduced pregnancy outcome in patients with unilateral or bilateral hydrosalpinx after in vitro fertilization.
  17. (1989). The effect of Rhesus uterine epithelial cell monolayers on in-vitro growth of Rhesus embryos. Theriogenology
  18. (1993). The search for improved in-vitro systems should not be ignored: embryo co-culture may be one of them. Hum Reprod