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Point mutation at codon 12 of the c-Ha-ras gene in human gastric cancers.

By E. H. Koh, H. C. Chung, K. B. Lee, E. K. Han, S. H. Oh, J. S. Min, E. M. Choi, J. K. Youn and B. S. Kim


The molecular mechanisms of the carcinogenic process of gastric cancer have not been fully understood yet. In order to know whether c-Ha-ras gene is being involved in the process of gastric carcinogenesis, 8 gastric cancer cell lines, 8 cases of gastric cancer and same number of adjacent dysplasia were analyzed for the presence of mutation at codon 12, 13 and 61 of the c-Ha-ras gene by using polymerase chain reaction (PCR) and mutant-specific oligonucleotide hybridization. Point mutations at codon 12 of the c-Ha-ras gene were found in 2 out of 8 gastric cancer and dysplasia samples in one case, but we found no mutation at codon 13 or 61 of the c-Ha-ras gene. These results suggest that the frequency of mutation of the c-Ha-ras gene detected by sensitive PCR technique is low indeed, however it would be notable that such a genetic change has been detected in the dysplastic lesion of the gastric cancer patient

Topics: Research Article
Publisher: Korean Academy of Medical Sciences
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Provided by: PubMed Central

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