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Life is sweet!: A novel role for N-glycans in Drosophila lifespan

By Harry Schachter and Gabrielle L Boulianne

Abstract

N-glycans are post-translational modifications in which the sugar chain is covalently linked to protein by a GlcNacβ1-N-asparagine linkage. Drosophila melanogaster and other invertebrates, but not vertebrates, synthesize large amounts of “paucimannose” N-glycans that contain only three or four mannose residues. The enzyme UD P-GlcNac:α3-D-mannoside β1,2-N-acetylglucosaminyltransferase I (GnTI, encoded by the Mgat1 gene) controls the synthesis of paucimannose N-glycans. Either deletion or neuron-specific knock-down of Mgat1 in wild-type flies results in pronounced defects in locomotion, structural defects in the adult central nervous system and a severely reduced lifespan. We have recently shown that neuronal expression of a wild-type Mgat1 transgene in Mgat1-null flies rescues the structural defects in the brain (fused β-lobes) and the shortened lifespan and, surprisingly, results in a dramatic 135% increase in mean lifespan relative to genetically identical controls that do not express the transgene. In this review, we discuss various approaches that can be used to determine the roles of paucimannose N-glycans in Drosophila longevity and in the adult CNS

Topics: Extra View
Publisher: Landes Bioscience
OAI identifier: oai:pubmedcentral.nih.gov:3052869
Provided by: PubMed Central
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