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Experimental infection of hamsters with avian paramyxovirus serotypes 1 to 9

By Arthur S Samuel, Madhuri Subbiah, Heather Shive, Peter L Collins and Siba K Samal


Avian paramyxoviruses (APMVs) are frequently isolated from domestic and wild birds throughout the world and are separated into nine serotypes (APMV-1 to -9). Only in the case of APMV-1, the infection of non-avian species has been investigated. The APMVs presently are being considered as human vaccine vectors. In this study, we evaluated the replication and pathogenicity of all nine APMV serotypes in hamsters. The hamsters were inoculated intranasally with each virus and monitored for clinical disease, pathology, histopathology, virus replication, and seroconversion. On the basis of one or more of these criteria, each of the APMV serotypes was found to replicate in hamsters. The APMVs produced mild or inapparent clinical signs in hamsters except for APMV-9, which produced moderate disease. Gross lesions were observed over the pulmonary surface of hamsters infected with APMV-2 & -3, which showed petechial and ecchymotic hemorrhages, respectively. Replication of all of the APMVs except APMV-5 was confirmed in the nasal turbinates and lungs, indicating a tropism for the respiratory tract. Histologically, the infection resulted in lung lesions consistent with bronchointerstitial pneumonia of varying severity and nasal turbinates with blunting or loss of cilia of the epithelium lining the nasal septa. The majority of APMV-infected hamsters exhibited transient histological lesions that self resolved by 14 days post infection (dpi). All of the hamsters infected with the APMVs produced serotype-specific HI or neutralizing antibodies, confirming virus replication. Taken together, these results demonstrate that all nine known APMV serotypes are capable of replicating in hamsters with minimal disease and pathology

Topics: Research
Publisher: BioMed Central
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  1. (1979). A further member of the Yucaipa group isolated from the common wren (Troglodytes troglodytes). Acta Virol
  2. (1938). A simple method of estimating fifty per cent endpoints.
  3. (2002). A summary of taxonomic changes recently approved by ICTV. Arch Virol
  4. (1983). Alexander DJ: Isolation of avian paramyxovirus other than Newcastle disease virus from commercial poultry in Great Britain. Vet Rec
  5. (1986). Antigenic relationships between avian paramyxoviruses. I. Quantitative characteristics based on hemagglutination and neuraminidase inhibition tests. Arch Virol
  6. (1979). Aronovici A: The isolation of yucaipa-like paramyxoviruses from epizootics of a respiratory disease in turkey poultry farms in Israel. Vet Rec
  7. (2003). Avian paramyxoviruses 2-9. 11 edition.
  8. (1982). Avian paramyxoviruses-other than Newcastle disease virus. World’s Poul Sci J
  9. (2005). BK: In Family Paramyxoviridae. Virus Taxonomy: The Classification and Nomenclature of Viruses.
  10. CA: Newcastle disease virus in man; results of studies in five cases. JL a bC l i nM e d1952,
  11. (1982). Characterization of avian paramyxoviruses isolated from feral ducks in northern Japan: the presence of three distinct viruses in nature. Microbiol Immunol
  12. (1982). Collins MS: Pathogenecity of PMV-3/Parakeet/Netherland/ 449/75 for chickens. Avian Pathol
  13. (2008). Collins PL: Newcastle disease virus as a vaccine vector for humans. Curr Opin Mol Ther
  14. (2005). Collins PL: Recombinant newcastle disease virus expressing a foreign viral antigen is attenuated and highly immunogenic in primates.
  15. (1999). Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae.
  16. (2008). E: Comparative study on the pathogenicity and immunogenicity of wild bird isolates of avian paramyxovirus 2, 4, and 6 in chickens. Avian Pathol
  17. (2008). E: Prevalence of antibodies to different avian paramyxoviruses in commercial poultry in the United States. Avian Dis
  18. (1979). Easterday BC: A hitherto unreported paramyxovirus of turkeys. Res Vet Sci
  19. (2003). EF: Investigation of several selected adjuvants regarding their efficacy and side effects for the production of a vaccine for parakeets to prevent a disease caused by a paramyxovirus type 3. Vaccine
  20. (1981). Effect of paramyxovirus yucaipa on fertility, hatchability, and poult yield of turkeys. Avian Dis
  21. (1950). Experimental inoculation of swine and sheep with Newcastle disease virus. Cornell Vet
  22. (1978). Fukumi H: Isolation of a new avian paramyxovirus from budgerigar (Melopsittacus undulatus).
  23. (1960). GT: Isolation of an unidentified agent from the respiratory tract of chickens. Science
  24. (2004). Guberti V: Isolation of an avian paramyxovirus type 9 from migratory waterfowl in Italy. Vet Rec
  25. (2001). HK: Complete nucleotide sequence of avian paramyxovirus type 6 isolated from ducks.
  26. (1981). Influenza A virus infection of domestic ducks first int. symp. avian influenza.
  27. (1952). Isolation of Newcastle disease virus from a calf. J Am Vet Med Assoc
  28. (2006). Isolation, identification, and comparison of four isolates of avian paramyxovirus serotype 2 in China. Avian Dis
  29. (2008). KS: Full-length genome sequence of avain paramyxovirus type 4 isolated from a mallard duck. Virus Genes
  30. (1987). Meddens MJ: Hamster model for herpes simplex virus infection of the central nervous system. Infection
  31. (2000). Newcastle disease and other avian paramyxoviruses. Rev Sci Tech
  32. (2007). Parks G: In Paramyxoviridae: the viruses and their replication. 5 edition. Edited by: Knipe
  33. (2008). Pekosz A: An immunosuppressed Syrian golden hamster model for SARS-CoV infection. Virology
  34. (1997). Prince GA: Animal models of respiratory syncytial virus infection. Clin Infect Dis
  35. (1968). Reynolde B: Avian influenza A and paramyxo viruses complicating respiratory disease diagnosis in poultry. Avian Dis
  36. (1952). RJ: An outbreak of conjunctivitis due to Newcastle disease virus (NDV) occurring in poultry workers.
  37. (1997). RN: Natural and experimental infection of turkeys with avian paramyxovirus-7. Avian Dis
  38. (1980). Rosenberger JK: Characterization of nine avian paramyxoviruses. Avian Dis
  39. (2010). Samal SK: Complete genome sequence of avian paramyxovirus (APMV) serotype 5 completes the analysis of nine APMV serotypes and reveals the longest APMV genome. PLoS One
  40. (2008). Samal SK: Complete genome sequence of avian paramyxovirus type 3 reveals an unusually long trailer region. Virus Res
  41. (2009). Samal SK: Complete genome sequence of avian paramyxovirus type 7 (strain Tennessee) and comparison with other paramyxoviruses. Virus Res
  42. (2009). Samal SK: Complete genome sequences of avian paramyxovirus type 8 strains goose/Delaware/1053/ 76 and pintail/Wakuya/20/78. Virus Res
  43. (2008). Samal SK: Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses. Virus Res
  44. (2009). Samal SK: Complete sequence of the genome of avian paramyxovirus type 9 and comparison with other paramyxoviruses. Virus Res
  45. (2008). Samal SK: Experimental infection of calves with Newcastle disease virus induces systemic and mucosal antibody responses. Arch Virol
  46. (2008). Samal SK: Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75. Virol J
  47. (1998). Samal SK: Nucleotide sequences of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence.
  48. (2004). Samal SK: Role of fusion protein cleavage site in the virulence of Newcastle disease virus. Microb Pathog
  49. (2004). Samal SK: The hemagglutinin-neuraminidase protein of Newcastle disease virus determines tropism and virulence.
  50. (2008). Samal SK: The large polymerase protein is associated with the virulence of Newcastle disease virus.
  51. (2004). SC: The hamster as an animal model for eastern equine encephalitis–and its use in studies of virus entrance into the brain. J Infect Dis
  52. (1978). SL: Isolation of Newcastle disease virus from Phasianidae birds in Hong Kong.
  53. (2004). TF: Nipah virus: vaccination and passive protection studies in a hamster model.
  54. (2008). WS: Immunosuppression enhances oncolytic adenovirus replication and antitumor efficacy in the Syrian hamster model. Mol Ther