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Copper Promotes the Trafficking of the Amyloid Precursor Protein*

By Karla M. Acevedo, Ya Hui Hung, Andrew H. Dalziel, Qiao-Xin Li, Katrina Laughton, Krutika Wikhe, Alan Rembach, Blaine Roberts, Colin L. Masters, Ashley I. Bush and James Camakaris


Accumulation of the amyloid β peptide in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer disease. Amyloid β peptide is generated from the sequential protease cleavage of the amyloid precursor protein (APP). We reported previously that copper increases the level of APP at the cell surface. Here we report that copper, but not iron or zinc, promotes APP trafficking in cultured polarized epithelial cells and neuronal cells. In SH-SY5Y neuronal cells and primary cortical neurons, copper promoted a redistribution of APP from a perinuclear localization to a wider distribution, including neurites. Importantly, a change in APP localization was not attributed to an up-regulation of APP protein synthesis. Using live cell imaging and endocytosis assays, we found that copper promotes an increase in cell surface APP by increasing its exocytosis and reducing its endocytosis, respectively. This study identifies a novel mechanism by which copper regulates the localization and presumably the function of APP, which is of major significance for understanding the role of APP in copper homeostasis and the role of copper in Alzheimer disease

Topics: Molecular Bases of Disease
Publisher: American Society for Biochemistry and Molecular Biology
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Provided by: PubMed Central
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