Article thumbnail

The Formation of the Bicoid Morphogen Gradient Requires Protein Movement from Anteriorly Localized mRNA

By Shawn C. Little, Gašper Tkačik, Thomas B. Kneeland, Eric F. Wieschaus and Thomas Gregor

Abstract

New quantitative data show that the Bicoid morphogen gradient is generated from a dynamic localized source and that protein gradient formation requires protein movement along the anterior-posterior axis

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3046954
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (1988). A gradient of bicoid protein in Drosophila embryos.
  2. (2002). A monomeric red fluorescent protein.
  3. (2008). A quantitative spatiotemporal atlas of gene expression in the Drosophila blastoderm.
  4. (2010). A two-dimensional simulation model of the bicoid gradient in Drosophila.
  5. (2002). A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
  6. (2009). Anteriorposterior positional information in the absence of a strong Bicoid gradient.
  7. (2008). Changes in bicoid mRNA anchoring highlight conserved mechanisms during the oocyte-to-embryo transition.
  8. (1994). Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs.
  9. (2009). Determining the scale of the Bicoid morphogen gradient.
  10. (1998). Developmental regulation of bicoid mRNA stability is mediated by the first 43 nucleotides of the 39 untranslated region.
  11. (2005). Diffusion and scaling during early embryonic pattern formation.
  12. (2001). Dimerization of the 39UTR of bicoid mRNA involves a two-step mechanism.
  13. (2010). Distinguishing direct from indirect roles for bicoid mRNA localization factors.
  14. (1997). Drosophila embryonic pattern repair: how embryos respond to bicoid dosage alteration.
  15. (2005). Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics.
  16. (2002). Establishment of developmental precision and proportions in the early Drosophila embryo.
  17. (2008). Fluorescent in situ hybridization protocols in Drosophila embryos and tissues.
  18. (2009). Formation of the bicoid morphogen gradient: an mRNA gradient dictates the protein gradient.
  19. (1996). From gradients to stripes in Drosophila embryogenesis: filling in the gaps.
  20. (2010). High mobility of bicoid captured by fluorescence correlation spectroscopy: implication for the rapid establishment of its gradient.
  21. (2008). Imaging individual mRNA molecules using multiple singly labeled probes.
  22. (2007). Incorporation of Drosophila CID/ CENP-A and CENP-C into centromeres during early embryonic anaphase.
  23. (2009). Lighting up mRNA localization in Drosophila oogenesis.
  24. (2006). Localization of bicoid mRNA in late oocytes is maintained by continual active transport.
  25. (2009). Localization, anchoring and translational control of oskar, gurken, bicoid and nanos mRNA during Drosophila oogenesis. Fly (Austin) 3: 15–28. Bicoid Gradient Formation Requires Protein Movement PLoS
  26. (2007). Master equation simulation analysis of immunostained Bicoid morphogen gradient.
  27. (2010). Minimizing offtarget signals in RNA fluorescent in situ hybridization.
  28. (2006). Modeling segmental patterning in Drosophila: maternal and gap genes.
  29. (2007). Modeling the bicoid gradient: diffusion and reversible nuclear trapping of a stable protein.
  30. (2010). Modelling the Bicoid gradient.
  31. (2005). Moving messages: the intracellular localization of mRNAs.
  32. (1989). Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte.
  33. (2010). Multiscale modeling of diffusion in the early Drosophila embryo.
  34. (2000). Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo.
  35. (1969). Positional information and the spatial pattern of cellular differentiation.
  36. (2005). Precise domain specification in the developing Drosophila embryo.
  37. (2007). Probing the limits to positional information.
  38. (2010). Quantifying the Bicoid morphogen gradient in living fly embryos. arXiv:1003.5572v2. Available at:
  39. (1991). RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos.
  40. (1997). RNA-RNA interaction is required for the formation of specific bicoid mRNA 39 UTRSTAUFEN ribonucleoprotein particles.
  41. (2004). Seeing is believing: the bicoid morphogen gradient matures.
  42. (2007). Stability and nuclear dynamics of the bicoid morphogen gradient.
  43. (2009). Stathopoulos A
  44. (1994). Staufen protein associates with the 39UTR of bicoid mRNA to form particles that move in a microtubule-dependent manner.
  45. (1991). Staufen, a gene required to localize maternal RNAs in the Drosophila egg.
  46. (1986). Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.
  47. (1983). Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis.
  48. (2009). Synchronous and stochastic patterns of gene activation in the Drosophila embryo.
  49. (2010). The Bicoid gradient is shaped independently of nuclei.
  50. (1988). The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner.
  51. (1989). The gradient morphogen bicoid is a concentration-dependent transcriptional activator.
  52. (1988). The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo.
  53. (2010). The time to measure positional information: maternal hunchback is required for the synchrony of the Bicoid transcriptional response at the onset of zygotic transcription.
  54. (1995). Understanding, improving and using green fluorescent proteins.
  55. (2009). Visualization of individual Scr mRNAs during drosophila embryogenesis yields evidence for transcriptional bursting.
  56. (1998). Visualization of single RNA transcripts in situ.