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Rapid PCR Detection of Staphylococcus aureus Clonal Complex 398 by Targeting the Restriction-Modification System Carrying sau1-hsdS1▿

By Marc Stegger, Jodi A. Lindsay, Arshnee Moodley, Robert Skov, Els M. Broens and Luca Guardabassi

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms

Topics: Clinical Veterinary Microbiology
Publisher: American Society for Microbiology
OAI identifier: oai:pubmedcentral.nih.gov:3043477
Provided by: PubMed Central
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