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A PCR test to detect the cereal root-knot nematode Meloidogyne naasi

By C. Zijlstra, R.A. van Hoof and D. Donkers-Venne


The cereal root-knot nematode Meloidogyne naasi can cause serious cereal crop losses. The nematode is also found in agricultural fields where non-host crops are grown. Control of M. naasi can be based on preventing its spread, host resistance and crop management as well as on the design of crop rotation systems. Detection methods are required for these purposes and can also be helpful for inspection services and experimental research. This study describes the development of a simple PCR test that enables the detection of M. naasi. Alignment of sequences of rDNA-ITS fragments of M. naasi and five other Meloidogyne species was used to design the M. naasi specific forward primer N-ITS. Together with the reverse primer R195 M. naasi specific amplification was achieve

Year: 2004
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Provided by: NARCIS
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