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HLA Genotyping and Antibody Characterization Using the Luminex™ Multiplex Technology

By Falko Markus Heinemann

Abstract

The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i.e. the definition of positive or negative antibody status) and antibody identification with high sensitivity and specificity. HLA typing at different levels of resolution with Luminex technology uses sequence-specific oligonucleotide probes bound to color-coded microbeads in order to identify HLA alleles encoded by the DNA sample. In general, the Luminex technology for histocompatibility analyses provides rapid sample processing in a 96-well format combined with limited technical complexity which means high cost efficiency

Topics: Review Article · Übersichtsarbeit
Publisher: S. Karger GmbH
OAI identifier: oai:pubmedcentral.nih.gov:2941832
Provided by: PubMed Central
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