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Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device

By Rupert S. W. Thomas, Peter D. Mitchell, Richard O. C. Oreffo and Hywel Morgan


We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population

Topics: Special Topic: Dielectrophoresis (Guest Editor: Ron Pethig)
Publisher: American Institute of Physics
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Provided by: PubMed Central
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