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PP2A-B’γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves

By Moona Rahikainen, Andrea Trotta, Sara Alegre, Jesus Pascual, Katariina Vuorinen, Kirk Overmyer, Barbara Moffatt, Stephane Ravanel, Erich Glawischnig and Saijaliisa Kangasjarvi

Abstract

Glucosinolates (GSL) of cruciferous plants comprise a major group of structurally diverse secondary compounds which act as deterrents against aphids and microbial pathogens and have large commercial and ecological impacts. While the transcriptional regulation governing the biosynthesis and modification of GSL is now relatively well understood, post-translational regulatory components that specifically determine the structural variation of indole glucosinolates have not been reported. We show that the cytoplasmic protein phosphatase 2A regulatory subunit B'gamma (PP2A-B'gamma) physically interacts with indole glucosinolate methyltransferases and controls the methoxylation of indole glucosinolates and the formation of 4-meth-oxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves. By taking advantage of proteomic approaches and metabolic analysis we further demonstrate that PP2A-B'gamma is required to control the abundance of oligomeric protein complexes functionally linked with the activated methyl cycle and the trans-methylation capacity of leaf cells. These findings highlight the key regulatory role of PP2A-B'gamma in methionine metabolism and provide a previously unrecognized perspective for metabolic engineering of glucosinolate metabolism in cruciferous plants

Topics: protein phosphatase 2A, glucosinolate, activated methyl cycle, Arabidopsis thaliana, L-HOMOCYSTEINE HYDROLASE, CYSTATHIONINE GAMMA-SYNTHASE, INDOLE-GLUCOSINOLATE, PLANT-CELLS, METHYLTRANSFERASE ACTIVITY, S-ADENOSYLHOMOCYSTEINE, METHIONINE METABOLISM, PROTEIN INTERACTIONS, CRYSTAL-STRUCTURES, STRESS RESPONSES, 1183 Plant biology, microbiology, virology, protein phosphatase 2A, glucosinolate, activated methyl cycle, Arabidopsis thaliana, L-HOMOCYSTEINE HYDROLASE, CYSTATHIONINE GAMMA-SYNTHASE, INDOLE-GLUCOSINOLATE, PLANT-CELLS, METHYLTRANSFERASE ACTIVITY, S-ADENOSYLHOMOCYSTEINE, METHIONINE METABOLISM, PROTEIN INTERACTIONS, CRYSTAL-STRUCTURES, STRESS RESPONSES, 1183 Plant biology, microbiology, virology
Publisher: Wiley Blackwell
Year: 2017
OAI identifier: oai:helda.helsinki.fi:10138/178883
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