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The Influence of Bovine Milk High or Low in Isoflavones on Hepatic Gene Expression in Mice

By Mette T. Skaanild and Tina S. Nielsen

Abstract

Isoflavones have generated much attention due to their potential positive effects in various diseases. Phytoestrogens especially equol can be found in bovine milk, as feed ration for dairy cows is comprised of plants containing phytoestrogens. The aim of this study was to analyze the changes in hepatic gene expression after dietary intake of milk high and low in isoflavones. In addition to pelleted feed female NMRI mice were offered water, water added either 17β-estradiol, equol, Tween 80, and milk high and low in isoflavone content for a week. Gene expression was analyzed using an array qPCR kit. It was revealed that Tween 80 and 17β-estradiol upregulated both phase I and phase II genes to the same extent whereas equol alone, high and low isoflavone milk did not alter the expression of phase I genes but decreased the expression of phase II genes. This study shows that dietary isoflavones can regulate the transcription of especially phase II liver enzymes which potentially could give rise to an increase in reactive oxygen metabolites that may contribute to the development of cancer

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:2905924
Provided by: PubMed Central

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Citations

  1. (2002). 17β-hydroxysteroid dehydrogenases and cancers,”
  2. (1957). A simple method for the isolation and purification of total lipides from animal tissues,”TheJournalofBiologicalChemistry,vol.226,no.1,pp. 497–509,
  3. (2007). Ag´ undez, “Polymorphisms of histamine-metabolizing enzymes and clinical manifestations of asthma and allergic rhinitis,” Clinical and Experimental Allergy,
  4. (2005). and preservation method on red clover isoflavone concentration,”
  5. (2004). Andr´ e, “New data regarding phytoestrogens content in bovine milk,”
  6. (1985). Copper amine oxidases and amines as regulators of cellular processes,”
  7. (2008). D a y ,P .A .F o s t e r ,H .J .T u t i l le ta l .
  8. (2006). Dietary flavonoids: effects on xenobiotic and carcinogen metabolism,”
  9. (1997). Differential effects of dietary phyto-oestrogens daidzein and equol on human breast cancer MCF-7 cells,”
  10. (2009). Estrogenic activity of bovine milk high or low in equol using immature mouse uterotrophic responses and an estrogen receptor transactivation assay,”
  11. (2007). Herbage and milk productivity and quality when grazing different legumes and herbs,”
  12. (2007). High levels of equol in organic skimmed Finnish cow milk,”
  13. (1995). Metabolism of estrogenic isoflavones in domestic animals,”
  14. (2007). P.Phrakonkham,J.Chevalier,C.Desmetzetal.,“Isoflavonoidbased bone-sparing treatments exert a low activity on reproductive organs and on hepatic metabolism of estradiol in ovariectomized rats,”
  15. (1997). Physiology and molecular genetics of 17β-hydroxysteroid dehydrogenases,”
  16. (1995). Phytoestrogen content and estrogenic effect of legume fodder,”
  17. (1999). Sandkuijl et al., “17β-hydroxysteroid dehydrogenase-3 deficiency: diagnosis, phenotypic variability, population genetics, and worldwide distribution of ancient and de novo mutations,”
  18. (2003). The OECD program to validate the rat uterotrophic bioassay. Phase 2: dietary phytoestrogen analyses,” Environmental Health Perspectives,
  19. (2004). The role of 17 beta-hydroxysteroid dehydrogenases,”