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High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam

By Le Thi Minh Vien, Stephen Baker, Le Thi Phuong Thao, Le Thi Phuong Tu, Cao Thu Thuy, Tran Thi Thu Nga, Nguyen Van Minh Hoang, James Iain Campbell, Lam Minh Yen, Nguyen Trong Hieu, Nguyen Van Vinh Chau, Jeremy Farrar and Constance Schultsz

Abstract

Antimicrobial-resistant pathogenic members of the Enterobacteriaceae are a well-defined global problem. We hypothesized that one of the main reservoirs of dissemination of antimicrobial resistance genes in Vietnam is non-pathogenic intestinal flora, and sought to isolate antimicrobial-resistant organisms from hospitalized patients and non-hospitalized healthy individuals in Ho Chi Minh City. The results identified substantial faecal carriage of gentamicin-, ceftazidime- and nalidixic acid-resistant members of the Enterobacteriaceae in both hospitalized patients and non-hospitalized healthy individuals. A high prevalence of quinolone resistance determinants was identified, particularly the qnrS gene, in both community- and hospital-associated strains. Furthermore, the results demonstrated that a combination of quinolone resistance determinants can confer resistance to nalidixic acid and ciprofloxacin, even in the apparent absence of additional chromosomal resistance mutations in wild-type strains and laboratory strains with transferred plasmids. These data suggest that intestinal commensal organisms are a significant reservoir for the dissemination of plasmid-mediated quinolone resistance in Ho Chi Minh City

Topics: Antimicrobial Agents and Chemotherapy
Publisher: Society for General Microbiology
OAI identifier: oai:pubmedcentral.nih.gov:2884939
Provided by: PubMed Central

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