Article thumbnail

Viral vector-mediated overexpression of α-synuclein as a progressive model of Parkinson's disease

By Ayse Ulusoy, Mickael Decressac, Deniz Kirik and Anders Björklund

Abstract

The discovery of the role of α-synuclein in the pathogenesis of Parkinson's disease (PD) has opened new possibilities for the development of more authentic models of Parkinson's disease. Recombinant adeno-associated virus (AAV) and lentivirus (LV) vectors are efficient tools for expression of genes locally in subsets of neurons in the brain and can be used to express human wild-type or mutated α-synuclein selectively in midbrain dopamine neurons. Using this approach, it is possible to trigger extensive PD-like cellular and axonal pathologies in the nigrostriatal projection, involving abnormal protein aggregation, neuronal dysfunction, and cell death that develop progressively over time. Targeted overexpression of human α-synuclein in midbrain dopamine neurons, using AAV vectors, reproduces many of the characteristic features of the human disease and provides, for the first time, a model of progressive PD that can be applied to both rodents and primates

Topics: Neurosciences, Synuclein, Dopamine, Nigrostriatal system, Motor impairment, Adeno-associated virus, Lentivirus, Viral vectors, Animal models
Publisher: 'Elsevier BV'
Year: 2010
DOI identifier: 10.1016/S0079-6123(10)84005-1
OAI identifier: oai:lup.lub.lu.se:084e1107-2d8f-4fce-bb2d-3425f6845593
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://dx.doi.org/10.1016/S007... (external link)
  • https://lup.lub.lu.se/record/1... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.