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Time-resolved metabolomics analysis of beta-cells implicates the pentose phosphate pathway in the control of insulin release

By Peter Spégel, Vladimir Sharoyko, Isabel Göhring, Anders Danielsson, Siri Malmgren, Cecilia Nagorny, Lotta Andersson, Thomas Köck, Geoffrey W. G. Sharp, Susanne G. Straub, Claes Wollheim and Hindrik Mulder

Abstract

Insulin secretion is coupled with changes in beta-cell metabolism. To define this process, 195 putative metabolites, mitochondrial respiration, NADP(+), NADPH and insulin secretion were measured within 15 mm of stimulation of clonal INS-1 832/13 beta-cells with glucose. Rapid responses in the major metabolic pathways of glucose occurred, involving several previously suggested metabolic coupling factors. The complexity of metabolite changes observed disagreed with the concept of one single metabolite controlling insulin secretion. The complex alterations in metabolite levels suggest that a coupling signal should reflect large parts of the beta-cell metabolic response. This was fulfilled by the NADPH/NADP(+) ratio, which was elevated (8-fold; P 2.5-fold; P < 0.001). Inhibition of the pentose phosphate pathway by trans-dehydroepiandrosterone (DHEA) suppressed ribose 5-phosphate levels and production of reduced glutathione, as well as insulin secretion in INS-1 832/13 beta-cells and rat islets without affecting ATP production. Metabolite profiling of rat islets confirmed the glucose-induced rise in ribose 5-phosphate, which was prevented by DHEA. These findings implicate the pentose phosphate pathway, and support a role for NADPH and glutathione, in beta-cell stimulus-secretion coupling

Topics: Biochemistry and Molecular Biology, glutathione, islets, mass spectrometry, NADPH, ribose 5-phosphate, Type, 2 diabetes
Publisher: 'Portland Press Ltd.'
Year: 2013
DOI identifier: 10.1042/BJ20121349
OAI identifier: oai:lup.lub.lu.se:512e5f02-915d-4894-aa1b-8c4ab1fd1d55
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