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Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

By Marian AC Piqueur, Walter A Verstrepen, Peggy Bruynseels and An H Mertens

Abstract

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample

Topics: Short Report
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:2716329
Provided by: PubMed Central

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Citations

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