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Production, purification, crystallization and preliminary X-ray diffraction analysis of the HIV-2-neutralizing V3 loop-specific Fab fragment 7C8

By Hannes Uchtenhagen, Samer Sourial, Rosmarie Friemann, Mariethe Ehnlund, Anna-Lena Spetz, Robert A. Harris, Chaithanya Madhurantakam and Adnane Achour

Abstract

Neutralizing Fab fragments of the HIV-2-binding murine antibody 7C8 were generated after purification from hybridoma cell-culture supernatant. Crystallization conditions were determined and diffraction data were collected to 2.7 Å resolution

Topics: Crystallization Communications
Publisher: International Union of Crystallography
OAI identifier: oai:pubmedcentral.nih.gov:2705640
Provided by: PubMed Central
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