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Regulation of Episomal Gene Expression by KRAB/KAP1-Mediated Histone Modifications▿

By Isabelle Barde, Elisa Laurenti, Sonia Verp, Anna Claire Groner, Christopher Towne, Viviane Padrun, Patrick Aebischer, Andreas Trumpp and Didier Trono

Abstract

KAP1 is an essential cofactor of KRAB zinc finger proteins, a family of vertebrate-specific epigenetic repressors of largely unknown functions encoded in the hundreds by the mouse and human genomes. So far, KRAB/KAP1-mediated gene regulation has been studied within the environment of chromosomal DNA. Here we demonstrate that KRAB/KAP1 regulation is fully functional within the context of episomal DNA, such as adeno-associated viral and nonintegrated lentiviral vectors, and is correlated with histone modifications typically associated with this epigenetic regulator

Topics: Gene Delivery
Publisher: American Society for Microbiology (ASM)
OAI identifier: oai:pubmedcentral.nih.gov:2681943
Provided by: PubMed Central
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