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Biodiversity of pig breeds from China and Europe estimated from pooled DNA samples: differences in microsatellite variation between two areas of domestication

By Hendrik-Jan Megens, Richard PMA Crooijmans, Magali San Cristobal, Xiao Hui, Ning Li and Martien AM Groenen


Microsatellite diversity in European and Chinese pigs was assessed using a pooled sampling method on 52 European and 46 Chinese pig populations. A Neighbor Joining analysis on genetic distances revealed that European breeds were grouped together and showed little evidence for geographic structure, although a southern European and English group could tentatively be assigned. Populations from international breeds formed breed specific clusters. The Chinese breeds formed a second major group, with the Sino-European synthetic Tia Meslan in-between the two large clusters. Within Chinese breeds, in contrast to the European pigs, a large degree of geographic structure was noted, in line with previous classification schemes for Chinese pigs that were based on morphology and geography. The Northern Chinese breeds were most similar to the European breeds. Although some overlap exists, Chinese breeds showed a higher average degree of heterozygosity and genetic distance compared to European ones. Between breed diversity was even more pronounced and was the highest in the Central Chinese pigs, reflecting the geographically central position in China. Comparing correlations between genetic distance and heterozygosity revealed that China and Europe represent different domestication or breed formation processes. A likely cause is a more diverse wild boar population in Asia, but various other possible contributing factors are discussed

Topics: Research
Publisher: BioMed Central
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Provided by: PubMed Central

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  1. (2004). 3: Integrated software for molecular evolutionary genetics analysis and sequence alignment,
  2. (1993). A Handbook to the Breeds of the World, Helm InformationLtd.,
  3. (1963). A History of Domesticated Animals, Harper and Row Publishers,
  4. (1999). A natural history of domesticated mammals, Second Edn.
  5. (1983). Accuracy of estimated phylogenetic trees from molecular data,
  6. (2004). Accurate determination of microsatellite allele frequencies in pooled DNA samples,
  7. (2003). An EC-funded project on characterization of genetic variation in the European pig. Objectives, organization, breed sampling, DNA preparation and circulation,
  8. (2003). Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools,
  9. (2006). CardellinoR., Geneticdiversitywithinand betweenEuropeanpig breeds using microsatellite markers,
  10. (1985). Confidence limits on phylogenies: an approach using the bootstrap,
  11. (2004). Estimating the frequency of Asian cytochromeB haplotypesin standardEuropeanand local Spanish breeds,Genet.
  12. (1983). Estimation of the coancestry coeffi-cient: basis for a short-term genetic distance,
  13. (2006). Evaluation of DNA Pooling for the estimation of microsatellite allele frequencies:a case study using Striped Bass (Morone saxatilis),
  14. (1998). Genetic aspects of domestication, common breeds and their origin, in: Ruvinsky A.,
  15. (1972). Genetic distance between populations,
  16. (1996). Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA,
  17. (2000). Genetic diversity of eleven pig breeds,
  18. (2003). Genetic diversity of microsatellite loci in fifty-six Chinese native pig breeds,
  19. (1998). Genetic parameters and genetic trends in the Sino-European Tia Meslan composite line,
  20. (2005). Genetic structure of pig breeds from Korea and China using microsatellite loci analysis,
  21. (2000). Genetic structure of the Iberian pig breed using microsatellites,
  22. (2003). Genetic variation and relationships of eighteen Chinese indigenous pig breeds,
  23. (2005). Genetical and mathematical propertiesof similarity and dissimilarity coefficients applied in plant breeding and seed bank management,
  24. (2003). Geneticcharacterization of Southwestern European bovine breeds: a historical and biogeographical reassessment with a set of 16 microsatellites,
  25. (1961). Handbuch der Tierzüchtung,Dritter Band Rassenkunde, Verlag Paul Parey,
  26. (2006). HuangM.-C.,Li L.-H.,Lin C.-H.,Wu J.-Y., ChenY.-T., Fann C.S.J., A genome-wide study of preferential amplification/hybridizationin micro-array-based pooled DNA experiments,
  27. (2004). Livestock Biodiversity. Genetic Resources for the Farming of the Future, Blackwell Science,
  28. (2002). Mason’s World Dictionary of Livestock Breeds,
  29. (2002). Measuring genetic distances between breeds:use ofsome distancesin variousshorttermevolutionmodels,Genet.Sel.
  30. (2006). Microsatellite analysis of pooled Schistosoma mansoniDNA: an approachfor studies of parasite populations,Parasitology 132
  31. (1996). Microsatellite polymorphism in commercial broiler and layer lines estimated using pooled blood samples,
  32. (2006). Mitochondrial diversity in European and Chinese pigs is consistent with population expansions that occurred prior to domestication,
  33. (1964). Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis,
  34. (2000). NTSYSpc 2.1, Numerical Taxonomy and Multivariate Analysis System,
  35. (2005). PHYLIP (Phylogeny Inference Package) version 3.6, Distributed by the author,
  36. (1967). Phylogenetic analysis: models and estimation procedures,
  37. (1966). Phylogenetic systematics (English trans. and extensively
  38. (1986). Pig Breeds
  39. (1999). RuaneJ.,Acriticalreviewofthevalueofgeneticdistancestudiesinconservation of animal genetic resources,
  40. (1990). The chance to survive,
  41. (1993). The Eurasian wild pig (Susscrofa), in: Pigs, Peccaries and Hippos: Status Surveyand Action Plan,
  42. (1994). The historyandgeographyof human genes,
  43. (1987). The neighbor-joiningmethod: a new method for reconstructing phylogenetic trees,
  44. (2000). The origin of the domestic pig: independentdomestication and subsequentintrogression,
  45. (2005). The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers,
  46. (2003). The use of microsatellite genotyping for population studies in the pig with individual and pooled samples,
  47. (1868). The variation of animals and plants under domestication,
  48. (2000). World Watch List forAnimalDiversity,3rdedn.,FAO,
  49. (2005). Worldwide phylogeographyof wild boar reveals multiple centers of pig domestication,

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