Skip to main content
Article thumbnail
Location of Repository

Does Phenoloxidase Contributed to the Resistance? Selection with Butane-Fipronil Enhanced its Activities from Diamondback Moths

By Shouzhu Liu, Hongtao Niu, Ting Xiao, Chaobin Xue, Zhongde Liu and Wanchun Luo

Abstract

Using microtitration method, the relationship between Phenoloxidase activity and the resistance of the diamondback moth Plutella xylostella (Linnaeus) to the novel insecticide butane-fipronil was determined in vitro. After selection of the tenth-generation by butane-fipronil, the resistance of the fourth instar larvae was increased 83.80-fold as compared to the susceptible strain. Phenoloxidase activity of the resistant strain (POr) was 1.29-fold higher than the susceptible one (POs). However, the Km and optimum pH values were similar in resistant and susceptible strains, which were 1.11 mM and 6.5, respectively. Both POr and POs have maximum stability at pH values less than 7.0, although POs was less stable at lower pH values than POr. In addition, the thermal stabilities of the two phenoloxidase were very similar. It is suggested that PO may play an important role in the increasing resistance of pests to pesticides

Topics: Article
Publisher: Bentham Open
OAI identifier: oai:pubmedcentral.nih.gov:2674291
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing theprinciple of proteindye binding.
    2. (2006). Behavioral change, physiological modification, and metabolic detoxification: mechanisms of insecticide resistance. Acta Entomol.
    3. (2007). Bioactivity of butenefipronil and its field efficacy against diamondback moth, Plutella xylostella (L.). Acta Phytophylacica Sin.,
    4. (1956). Biology of the diamondbackmoth, Plutella maculipennis (Curt.) (Lepidoptera: Plutellidae) in eastern Ontario. I. Distribution, economic history, synonomy and general description. 37th Report of the Quebec Society for the Protection of Plants.
    5. (1991). Cross-resistance between acylurea insect grossth regulators in a strain of Plutella xylostella (L.)(Lepidoptera:
    6. (2004). Cross-resistance potential of fipronil in Musca domestica. Pest Manag. Sci.,
    7. (1997). Determination of phenoloxidase activity from fifth instar larvae of Bombyx mori.
    8. (1992). Effect of chlorfluazuron on the phenoloxidase and chitinase activity in Ostrinia furnacalis. Acta Entomol.
    9. (2006). Enzymatic properties of phenoloxidase from Pieris rapae (Lepidoptera) larvae. Insect Sci.,
    10. (2004). Fipronil resistance in the diamondback moth (Lepidoptera: Plutellidae): inheritance and number of genes involved.
    11. (2007). Fipronil resistance mechanisms in the rice stem borer, Chilo suppressalis
    12. (1992). Fipronil: A new soil and foliar broad spectrum insecticide.
    13. (1998). Fipronil: action at the GABA receptor.
    14. (1999). Genetic and biochemical mechanisms limiting fipronil toxicity in the LPR strain of house fly. Musca domestica.
    15. (2004). Genetics of spinosad resistance in a multi-resistant field-selected population of Plutella xylostella. Pest Manag. Sci.,
    16. (2005). Immune function responds to selection for cuticular colour in Tenebrio molitor. Heredity,
    17. (2006). Immune responses of locusts to challenge with the pathogenic fungus metarhizium or high doses of laminarin.
    18. (2005). Inhibitory effects of 4-dodecylresorcinol on the phenoloxidase of the diamondback moth enzymatic properties of Plutella xylostella (L.) (Lepidoptera plutellidae).
    19. (1995). Innate immunity of insects.
    20. (1996). Insect immunity: the haemocytes of the armyworm Mythimna unipuncta (Lepidoptera: Noctuidae) and their role in defence reactions. In vivo and in vitro studies.
    21. (1968). Insecticide resistance in the housefly: decreased rate of absorption as the mechanism of action of a gene that act as an intensifier of resistance.
    22. (2001). Insecticide resistance. In: Encyclopedia of Biodiversity;
    23. (1996). Insecticide susceptibility and detoxication enzyme activities in permethrin-selected diamondback moths.
    24. Interaction between the factor delaying penetration of insecticides and the demethylation mechanisms of resistance to organophosphorus-resistant houseflies.
    25. (2005). Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning.
    26. (2003). Local variation in susceptibility of thediamonback moth Plutella oxylostella Linnaeus to insecticides and role of detoxification enzymes. Crop Prot.,
    27. (1979). Method for diamond back moth (Plutella xylostella L.). FAO Plant Prot.,
    28. (2006). Model reactions for insect cuticle sclerotization: Cross-linking of recombinant cuticular proteins upon their laccase-catalyzed oxidative conjugation with catechols. Insect Biochem.
    29. (2005). Mosquitophenoloxidase and defensin colocalize in melanization innate immune responses.
    30. (2007). Physiological effect of quercetin on phenoloxidase from Tenebrio molitor. Acta Entomol.
    31. (1995). Role of the integument in insect defense: pro-phenol oxidase cascade in the cuticular matrix.
    32. (2005). Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth,
    33. (1998). Study on the effect of monocrotophos on activity of PO in haemolymph of Penaeus chinensis. Marine Sci.,

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.