Skip to main content
Article thumbnail
Location of Repository

DNA uracil repair initiated by the archaeal ExoIII homologue Mth212 via direct strand incision

By Lars Schomacher, James P. J. Chong, Paul McDermott, Wilfried Kramer and Hans-Joachim Fritz


No genes for any of the known uracil DNA glycosylases of the UDG superfamily are present in the genome of Methanothermobacter thermautotrophicus ΔH, making it difficult to imagine how DNA-U repair might be initiated in this organism. Recently, Mth212, the ExoIII homologue of M. thermautotrophicus ΔH has been characterized as a DNA uridine endonuclease, which suggested the possibility of a novel endonucleolytic entry mechanism for DNA uracil repair. With no system of genetic experimentation available, the problem was approached biochemically. Assays of DNA uracil repair in vitro, promoted by crude cellular extracts, provide unequivocal confirmation that this mechanism does indeed operate in M. thermautotrophicus ΔH

Topics: Genome Integrity, Repair and Replication
Publisher: Oxford University Press
OAI identifier:
Provided by: PubMed Central

Suggested articles


  1. (1995). A eukaryotic gene encoding an endonuclease that specifically repairs DNA damaged by ultraviolet light.
  2. (1998). A novel DNA polymerase family found in Archaea.
  3. (2004). A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system.
  4. (2002). A novel type of uracil-DNA glycosylase mediating repair of hydrolytic DNA damage in the extremely thermophilic eubacterium Thermus thermophilus.
  5. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
  6. (2005). A recombinant exonuclease III homologue of the thermophilic archaeon Methanothermobacter thermautotrophicus.
  7. (2000). Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base excision repair: the 30 ends justify the means.
  8. (1996). An unusual mechanism for the major human apurinic/apyrimidinic (AP) endonuclease involving 50 cleavage of DNA containing a benzene-derived exocyclic adduct in the absence of an AP site.
  9. (1996). Characterization of a UV endonuclease gene from the fission yeast Schizosaccharomyces pombe and its bacterial homolog.
  10. (1999). Comparison of the 50 nuclease activities of Taq DNA polymerase and its isolated nuclease domain.
  11. (1997). Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.
  12. (2004). Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis.
  13. (1998). Differential cleavage of oligonucleotides containing the benzene-derived adduct, 1,N6-benzetheno-dA, by the major human
  14. (2007). Ein neu entdeckter Weg der Reparatur hydrolytisch gescha ¨ digter DNA-Cytosinreste, etabliert im thermophilen Archaeon Methanothermobacter thermautotrophicus H. University of Go ¨ttingen, Doctoral thesis.
  15. (2003). Enzymology of base excision repair in the hyperthermophilic archaeon Pyrobaculum aerophilum.
  16. (1992). Fast quantitative assay of sequence-specific endonuclease activity based on DNA sequencer technology.
  17. (1995). Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant.
  18. (1986). Hydrolysis by restriction endonucleases at their DNA recognition sequences substituted with mismatched base pairs.
  19. (1993). Instability and decay of the primary structure of DNA.
  20. (1999). Isolation and characterization of a split B-type DNA polymerase from the archaeon Methanobacterium thermoautotrophicum deltaH.
  21. (1983). Methyl-directed repair of DNA base-pair mismatches in vitro.
  22. (2007). OligoCalc: an online oligonucleotide properties calculator.
  23. (2000). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
  24. (1999). Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes.
  25. (1986). Purification and characterization of DNA polymerase from the archaebacterium Methanobacterium thermoautotrophicum.
  26. (1996). Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein.
  27. (1994). Reconstitution of the DNA base excision-repair pathway.
  28. (1997). Second pathway for completion of human DNA base excision-repair: reconstitution with purified proteins and requirement for DNase IV (FEN1).
  29. (2004). Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair.
  30. (2000). Structure and function in the uracil-DNA glycosylase superfamily.
  31. (1995). Substrate preferences of Vsr DNA mismatch endonuclease and their consequences for the evolution of the Escherichia coli K-12 genome.
  32. (1995). The base excision repair pathway.
  33. (2002). The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.
  34. (2006). The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis.
  35. (2006). The Methanothermobacter thermautotrophicus ExoIII homologue Mth212 is a DNA uridine endonuclease.
  36. (1991). The vsr gene product of E. coli K-12 is a strand- and sequence-specific DNA mismatch endonuclease.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.