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The Family X DNA Polymerase from Deinococcus radiodurans Adopts a Non-standard Extended Conformation

By Nicolas Leulliot, Lionel Cladière, François Lecointe, Dominique Durand, Ulrich Hübscher and Herman van Tilbeurgh

Abstract

Deinococcus radiodurans is an extraordinarily radioresistant bacterium that is able to repair hundreds of radiation-induced double-stranded DNA breaks. One of the players in this pathway is an X family DNA polymerase (PolXDr). Deletion of PolXDr has been shown to decrease the rate of repair of double-stranded DNA breaks and increase cell sensitivity to gamma-rays. A 3′→5′ exonuclease activity that stops cutting close to DNA loops has also been demonstrated. The present crystal structure of PolXDr solved at 2.46-Å resolution reveals that PolXDr has a novel extended conformation in stark contrast to the closed “right hand” conformation commonly observed for DNA polymerases. This extended conformation is stabilized by the C-terminal PHP domain, whose putative nuclease active site is obstructed by its interaction with the polymerase domain. The overall conformation and the presence of non standard residues in the active site of the polymerase X domain makes PolXDr the founding member of a novel class of polymerases involved in DNA repair but whose detailed mode of action still remains enigmatic

Topics: DNA: Replication, Repair, Recombination, and Chromosome Dynamics
Publisher: American Society for Biochemistry and Molecular Biology
OAI identifier: oai:pubmedcentral.nih.gov:2673268
Provided by: PubMed Central
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