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Microdissection of Shoot Meristem Functional Domains

By Lionel Brooks, Josh Strable, Xiaolan Zhang, Kazuhiro Ohtsu, Ruilian Zhou, Ananda Sarkar, Sarah Hargreaves, Robert J. Elshire, Douglas Eudy, Teresa Pawlowska, Doreen Ware, Diane Janick-Buckner, Brent Buckner, Marja C. P. Timmermans, Patrick S. Schnable, Dan Nettleton and Michael J. Scanlon


The shoot apical meristem (SAM) maintains a pool of indeterminate cells within the SAM proper, while lateral organs are initiated from the SAM periphery. Laser microdissection–microarray technology was used to compare transcriptional profiles within these SAM domains to identify novel maize genes that function during leaf development. Nine hundred and sixty-two differentially expressed maize genes were detected; control genes known to be upregulated in the initiating leaf (P0/P1) or in the SAM proper verified the precision of the microdissections. Genes involved in cell division/growth, cell wall biosynthesis, chromatin remodeling, RNA binding, and translation are especially upregulated in initiating leaves, whereas genes functioning during protein fate and DNA repair are more abundant in the SAM proper. In situ hybridization analyses confirmed the expression patterns of six previously uncharacterized maize genes upregulated in the P0/P1. P0/P1-upregulated genes that were also shown to be downregulated in leaf-arrested shoots treated with an auxin transport inhibitor are especially implicated to function during early events in maize leaf initiation. Reverse genetic analyses of asceapen1 (asc1), a maize D4-cyclin gene upregulated in the P0/P1, revealed novel leaf phenotypes, less genetic redundancy, and expanded D4-CYCLIN function during maize shoot development as compared to Arabidopsis. These analyses generated a unique SAM domain-specific database that provides new insight into SAM function and a useful platform for reverse genetic analyses of shoot development in maize

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    1. (1759) Theoria Generationis, Halae ad Salam, Litteris Hendelianis.
    2. (2008). 5. [Epub ahead of print].
    3. (1992). A dominant mutation in the maize homeobox gene, Knotted-1, causes its ectopic expression in leaf cells with altered fates.
    4. (1992). A fate map of the Arabidopsis embryonic shoot apical meristem.
    5. (1996). A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis.
    6. (1998). AGO1 defines a novel locus of Arabidopsis controlling leaf development.
    7. (2003). Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3.
    8. (2001). Analysis and expression of the alphaexpansin and beta-expansin gene families in maize.
    9. (2005). Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs.
    10. (2007). Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses.
    11. (2004). Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities.
    12. (2006). ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis.
    13. (2000). Auxin regulates the initiation and radial position of plant lateral organs.
    14. (1999). CDK inhibitors: positive and negative regulators of G1-phase progression.
    15. (1984). Cellular parameters of leaf morphogenesis in maize and tobacco.
    16. (1978). Characterization of a mutator system in maize.
    17. (1995). Cloning and characterization of the maize An1 gene.
    18. (2004). Computational identification of plant microRNAs and their targets, including a stress-induced miRNA.
    19. (2004). Control of phyllotaxy by the cytokinininducible response regulator homologue ABPHYL1.
    20. (1999). Control of phyllotaxy in maize by the abphyl1 gene.
    21. (1995). Controlling false discovery rate: a practical and powerful approach to multiple testing.
    22. (2000). Cyclin D control of growth rate in plants.
    23. (2005). Cyclin D regulation of a sexually dimorphic asymmetric cell division.
    24. (2007). Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase.
    25. (2002). Empty pericarp2 encodes a negative regulator of the heat shock response and is required for maize embryogenesis.
    26. (1995). Expansins in growing tomato leaves.
    27. (1999). Expression of an amino acid biosynthesis gene in tomato flowers: developmental upregulation and MeJa response are parenchyma-specific and mutually compatible.
    28. (2006). Expression of maize D-type cyclins: comparison, regulation by phytohormones during seed germination and description of a new D cyclin.
    29. (2000). FACKEL is a sterol C-14 reductase required for organized cell division and expansion in Arabidopsis embryogenesis.
    30. (2004). Genome-wide analysis of the cyclin family in Arabidopsis and comparative phylogenetic analysis of plant cyclin-like proteins.
    31. (2007). Genomic Organization and Evolutionary Conservation of Plant D-Type Cyclins.
    32. (2007). Global gene expression analysis of the shoot apical meristem of maize (Zea mays L.).
    33. (1999). Gnarley1 is a dominant mutation in the knox4 homeobox gene affecting cell shape and identity.
    34. (1991). In situ hybridization in plants. In: Molecular Plant Pathology: A Practical Approach.
    35. (2007). Involving undergraduates in the annotation and analysis of global gene expression studies: creation of a maize shoot apical meristem expression database.
    36. (2005). Isolation, characterization, and pericycle-specific transcriptome analyses of the novel maize lateral and seminal root initiation mutant rum1.
    37. (2005). Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis.
    38. (2007). Laser microdissection of narrow sheath mutant maize uncovers novel gene expression in the shoot apical meristem.
    39. (2006). Laser microdissection of plant tissue: what you see is what you get.
    40. (2003). Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: Identification of genes expressed differentially in epidermal cells or vascular tissues of maize.
    41. (2007). Leaf polarity: the role of small RNAs and positional signals in the shoot apex.
    42. (1998). Leafbladeless1 is required for dorsoventrality of lateral organs in maize.
    43. (2000). LEUNIG, a putative transcriptional corepressor that regulates AGAMOUS expression during flower development.
    44. (2004). limmaGUI: a graphical user interface for linear modeling of microarray data.
    45. (2004). Linear models and empirical Bayes methods for assessing differential expression in microarray experiments.
    46. (2003). Maize-targeted mutagenesis, A knockout resource for maize.
    47. (2008). maturation traits and auxin activity: Implications for somatic embryogenesis.
    48. (2004). microRNAmediated repression of rolled leaf1 specifies maize leaf polarity.
    49. (2008). Multiple MONOPTEROS-dependent pathways are involved in leaf initiation.
    50. (2003). Normalization of cDNA microarray data.
    51. (2002). OsPNH1 regulates leaf development and maintenance of the shoot apical meristem in rice.
    52. (1989). Patterns in Plant Development. Cambridge:
    53. (2008). Plant D-type cyclins: structure, roles and functions.
    54. (2007). Post-transcriptional and post-translational regulations of gene expression during leaf polarity formation.
    55. (2005). Radial leaves of the maize mutant ragged seedling2 retain dorsiventral anatomy.
    56. (2004). Real-time lineage analysis reveals oriented cell divisions associated with morphogenesis at the shoot apex of Arabidopsis thaliana.
    57. (2004). Regulation of flowering time in Arabidopsis by K homology domain proteins.
    58. (1998). Regulation of leaf initiation by the terminal ear 1 gene of maize.
    59. (2003). Regulation of phyllotaxis by polar auxin transport.
    60. (2009). Regulation of small RNA accumulation in the maize shoot apex.
    61. (2007). Rice ABERRANT PANICLE ORGANIZATION 1, encoding an F-box protein, regulates meristem fate.
    62. (2004). RNA silencing genes control de novo DNA methylation.
    63. (1998). Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis.
    64. (1999). ROUGH SHEATH2: a Myb protein that represses knox homeobox genes in maize lateral organ primordia.
    65. (2002). SEMAPHORE1 functions during the regulation of ancestrally duplicated knox genes and polar auxin transport in maize.
    66. (2000). Shoot meristem size is dependent on inbred background and presence of the maize homeobox gene, knotted1.
    67. (2004). Spatially restricted microRNA directs leaf polarity through ARGONAUTE1.
    68. (2007). Stomatal development.
    69. (2004). The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development.
    70. (2007). The Arabidopsis D-type cyclin CYCD4 controls cell division in the stomatal lineage of the hypocotyl epidermis.
    71. (2003). The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis.
    72. (2006). The D-type cyclin CYCD3;1 is limiting for the G1-to-S-phase transition in Arabidopsis.
    73. (2004). The dominant mutant Wavy auricle in blade1 disrupts patterning in a lateral domain of the maize leaf.
    74. (2000). The expression of D-cyclin genes defines distinct developmental zones in snapdragon apical meristems and is locally regulated by the Cycloidea gene.
    75. (2004). The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems.
    76. (1996). The maize mutant narrow sheath fails to establish leaf margin identity in a meristematic domain.
    77. (1999). The maize rough sheath2 gene and leaf development programs in monocot and dicot plants.
    78. (2008). The maize Viviparous8 locus, encoding a putative AMP1-like peptidase, regulates ABA accumulation and coordinates embryo and endosperm development.
    79. (2008). The milkweed pod1 gene encodes a KANADI protein that is required for abaxial/adaxial patterning in maize leaves.
    80. (1999). The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene.
    81. (2003). The polar auxin transport inhibitor N-1-naphthylphthalamic acid disrupts leaf initiation, KNOX protein regulation, and formation of leaf margins in maize.
    82. (2005). The role of ARGONAUTE1 (AGO1) in meristem formation and identity.
    83. (1998). The rough sheath2 gene negatively regulates homeobox gene expression during maize leaf development.
    84. (2004). Theologis A
    85. (2003). Three Brick genes have distinct functions in a common pathway promoting polarized cell division and cell morphogenesis in the maize leaf epidermis.
    86. (2007). Two small regulatory RNAs establish opposing fates of a developmental axis.
    87. (2006). UFO in the Arabidopsis inflorescence apex is required for floral-meristem identity and bract suppression.
    88. (2006). ZmPIN1a and ZmPIN1b encode two novel putative candidates for polar auxin transport and plant architecture determination of maize.
    89. (2003). ZWILLE buffers meristem stability in Arabidopsis thaliana.

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