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Maternal Smoking, GSTM1 and GSTT1 Polymorphism and Susceptibility to Adverse Pregnancy Outcomes

By Regina Grazuleviciene, Asta Danileviciute, Ruta Nadisauskiene and Jone Vencloviene


The objective of the study was to investigate the association between maternal smoking, GSTM1, GSTT1 polymorphism, low birth weight (LBW, < 2,500 g) and intra-uterine growth restriction (IUGR, < 2,500 g and gestation ≥ 37 weeks) risk. Within a prospective cohort study in Kaunas (Lithuania), a nested case-control study on LBW and IUGR occurrence among 646 women with genotyping of GSTT1 and GSTM1 polymorphisms who delivered live singletons was conducted. Multivariate logistic regression analysis was used to study the association of maternal smoking and polymorphism in two genes metabolizing xenobiotics. Without consideration of genotype, light-smoking (mean 4.8 cigarettes/day) during pregnancy was associated with a small increase in LBW risk, adjusted OR 1.21; 95% CI 0.44 – 3.31. The corresponding odds for IUGR risk was 1.57; 95% CI 0.45 – 5.55. The findings suggested the greater LBW risk among light-smoking mothers with the GSTM1-null genotype (OR 1.91; 95% CI 0.43 – 8.47) compared to those with GSTM1-present genotype (OR 1.11; 95% CI 0.26 – 4.47). When both GSTM1 and GSTT1 genotypes were considered, the synergistic effect was found among smoking mothers: GSTT1-present and GSTM1-null genotype OR for LBW was 3.31; 95% CI 0.60–18.4 and that for IUGR was 2.47; 95% CI 0.31 – 13.1. However there was no statistically significant interaction between maternal smoking, GSTT1- present and GSTM1-null genotypes for LBW (OR 1.45; 95% CI 0.22 – 10.1, p = 0.66) and for IUGR (OR 1.10; 95% CI 0.10 – 12.6, p = 0.93). The results of this study suggested that smoking, even at a low-level, ought to be considered a potential risk factor for adverse birth outcomes and that genetic polymorphism may contribute to individual variation in tobacco smoke response

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    1. (1996). A multiplex polymerase chain reaction protocol for the simultaneous analysis of the glutathione S-transferase GSTM1 and GSTT1 polymorphisms.
    2. (2008). Adverse birth outcomes associated with maternal smoking and polymorphisms in the N-nitrosamine-metabolizing enzyme genes NQO1 and CYP2E1.
    3. (2004). Agency for Research on Cancer). Tobacco smoking and involuntary smoking; ARC Monographs on the Evaluation of Carcinogenic Risks to Humans;
    4. (2006). Association of NDA adducts and genotypes with birth weight.
    5. (1993). Birth weight and perinatal mortality: the effect of maternal smoking.
    6. (2005). California Environmental Protection Agency. Proposed identification of environmental tobacco smoke as a toxic air contaminant: Part B health effects; California Environmental Protection Int.
    7. (2005). Correlates of cigarette smoking among school-going adolescents in Thailand: findings from the Thai global youth tobacco survey
    8. (1996). DNA adducts in human carcinogenesis: etiological relevance and structure–activity relationship.
    9. (2003). Effects of the GSTM1 and GSTT1 polymorphisms on the relationship between maternal exposure to environmental tobacco smoke and neonatal birth weight.
    10. (2007). Elevated risks of pregnancy complications and adverse outcomes with increasing maternal
    11. (2008). Environmental exposures and adverse pregnancy outcomes: a review of the science.
    12. (2007). Environmental exposures and gene regulation in disease etiology. Environ. Health Perspect.
    13. (2007). Ethnic differences in the association of birth weight and blood
    14. (1979). Exact conditional tests for cross-classifications: approximation of attained significance levels. Psychometrika
    15. (2008). Exploiting geneenvironment interaction to detect adverse health effects of environmental chemicals on the next generation. Basic Clin.
    16. (2000). Failure to defend a successful state tobacco control program: policy lesson.
    17. (2008). Gene-environment interactions in the development of complex disease phenotypes.
    18. (2000). Glutathione S-transferase polymorphisms and their biological consequences. Pharmacology
    19. (2009). Health impacts of long-term exposure to disinfection by-products in drinking water in Europe:
    20. (2000). Impact of perceived stress, major life events and pregnancy attitudes on low birth
    21. (2000). Influence of maternal active and passive smoking during pregnancy on birthweight in newborns.
    22. (1987). Intrauterine growth and gestational duration determinants. Pediatrics
    23. (2005). Low birth weight and preterm births: Etiologic fraction attributable to prenatal drug
    24. (2008). Maternal cigarette smoking, metabolic gene polymorphisms, and preterm delivery: new insights on GxE interactions and pathogenic
    25. (2002). Maternal cigarette smoking. Metabolic gene polymorphism and infant birth weight. JAMA
    26. (2008). Maternal exposure to environmental tobacco smoke, GSTM1/T1 polymorphism and oxidative stress.
    27. (2000). Maternal smoking and adverse birth outcomes among singletons and twins.
    28. (2006). Maternal smoking during pregnancy and genetic polymorphisms in the Ah receptor, CYP1A1 and GSTM1 affect infant birth size in Japanese subjects.
    29. (2004). Maternal/newborn GSTT1 null genotype contributes to risk of preterm, low birthweight infants. Pharmacogenetics
    30. (1995). Metabolic polymorphism affecting DNA binding and excretion of carcinogens in humans. Pharmacogenetics
    31. (2001). Office of the Surgeon General. Women and smoking: a report of the Surgeon General; Department of Health and Human Services:
    32. Pharmacogenomics of xenobiotic metabolizing enzymes in south American populations.
    33. (1998). Polycyclic aromatic hydrocarbon-DNA adducts in humans: relevance as biomarkers for exposure and cancer
    34. (2000). Prenatal active or passive tobacco smoke exposure and the risk of preterm delivery or low birth weight. Epidemiology
    35. (2008). Risk factors for low birth weight infants of Hispanic, African American, and white women in Bexar county, Texas. Pub. Health Nurs.
    36. (2003). Second hand smoke stimulates tumor angiogenesis and growth. Cancer Cell
    37. (2001). Smoking and pregnancy outcome among African-American and white women in central North Carolina. Epidemiology
    38. (2000). The impact of polycyclic aromatic hydrocarbons and fine particles on pregnancy outcome. Environ. Health Perspect.
    39. (2006). The role of cytochrome P450 enzymes in endogenous signaling pathways and environmental carcinogenesis.
    40. (2006). Xenobiotic-metabolizing genes and small-forgestational-age births: interaction with maternal smoking. Epidemiology

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