Skip to main content
Article thumbnail
Location of Repository

AFM and Multiple Transmission-Reflection Infrared Spectroscopy (MTR-IR) Studies on Formation of Air-Stable Supported Lipid Bilayers

By Peng-Feng Guo, Wen-Yi Huang, Hong-Bo Liu and Shou-Jun Xiao


Supported lipid bilayers (SLBs) were prepared by deposition of unilamellar vesicles on a silicon substrate. Atomic force microscopy (AFM) and a new Multiple Transmission-Reflection Infrared Spectroscopy (MTR-IR) developed by us were used to trace the dynamic formation of lipid bilayers on the silicon surfaces. The evolution from deformation of vesicles to formation of bilayers can be distinguished clearly by AFM imaging. MTR-IR provided high quality infrared spectra of ultrathin lipid bilayers with high sensitivity and high signal to noise ratio (SNR). The structural and orientational changes during vesicle’s fusion were monitored with MTR-IR. MTR-IR shows superiority over other infrared approaches for ultrathin films on standard silicon wafers in view of its economy and high sensitivity. Both MTR-IR and AFM results were consistent with each other and they provided more information for understanding the self-assembling procedure of SLBs

Topics: Article
Publisher: Molecular Diversity Preservation International (MDPI)
OAI identifier:
Provided by: PubMed Central

Suggested articles


  1. (2006). A multitechnique study of liposome adsorption on Au and lipid bilayer formation on SiO2. Langmuir
  2. (2001). A new approach to the IR spectroscopic study of molecular orientation and packing in adsorbed monolayers. Orientation and packing of long-chain primary amines and alcohols on quartz.
  3. (1977). A simple method for the preparation of homogeneous phospholipid vesicles. Biochemistry
  4. (1995). Atomic force microscopy of cholera toxin boligomers bound to bilayers of biologically relevant lipids.
  5. (2006). Automated formation of lipid-bilayer membranes in a microfluidic device. Nano Lett.
  6. (2006). Biosensing using lipid bilayers suspended on porous silicon. Langmuir
  7. (2003). Characterization of lipid bilayers and protein assemblies supported on rough surfaces by atomic force microscopy. Langmuir
  8. (2005). Cholesterol modulated antibody binding in supported lipid membranes as determined by total Internal reflectance microscopy on a microfabricated high-throughput glass chip. Langmuir
  9. (1992). Dynamic contact angles and deposition efficiency for transfer of docosanoic acid on to mica from CdCl2 subphases as a function of pH. Thin Solid Films
  10. (1996). Elaboration and characterization of phospholipid langmuir-blodgett films. Langmuir
  11. (1995). Electric field-induced concentration gradients in planar supported bilayers.
  12. (2005). Electrochemical and PM-IRRAS studies of the effect of the static electric field on the structure of the DMPC bilayer supported at a Au(111) electrode surface. Langmuir
  13. (2005). Following the formation of supported lipid bilayers on mica: A study combining
  14. (1996). Formation of membrane domains during the activation of protein kinase C. Biochemistry
  15. (2003). Formation of supported lipid bilayer membranes on SiO2 from proteoliposomes containing transmembrane proteins. Langmuir
  16. (2000). Formation of supported phospholipid bilayers from unilamellar vesicles investigated by atomic force microscopy. Langmuir
  17. (1992). Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers.
  18. (2000). From liposomes to supported, planar bilayer structures on hydrophilic and hydrophobic surfaces: An atomic force microscopy study.
  19. (2000). Gratton giant phospholipid vesicles: comparison among the whole lipid sample characteristics using different preparation methods: A two photon fluorescence microscopy study.
  20. (2005). Gravimetric antigen detection utilizing antibody-modified lipid bilayers.
  21. (2008). Influence of nanotopography on phospholipid bilayer formation on silicon dioxide.
  22. (1997). Kinetics of the unrolling of small unilamellar phospholipid vesicles onto self-assembled monolayers. Langmuir
  23. (1993). Langmuir-blodgett deposition of yttrium arachidate. Thin Solid Films
  24. (2005). Localized surface plasmon resonance sensing of lipid-membrane-mediated biorecognition events.
  25. (2005). Membrane on a chip: A functional tethered lipid bilayer membrane on silicon oxide surfaces.
  26. (2006). Microchannel device using selfspreading lipid bilayer as molecule carrier. Lab Chip
  27. (2008). Multiple transmissionreflection infrared spectroscopy for high-sensitivity measurement of molecular monolayers on silicon surfaces.
  28. (2007). PM IRRAS investigation of thin silica films deposited on gold. Part 1. Theory and proof of concept. Langmuir
  29. (2003). Probing lipid mobility of raftexhibiting model membranes by fluorescence correlation spectroscopy.
  30. (2004). Probing small unilamellar egg PC vesicles on mica surface by atomic force microscopy.
  31. (2000). Pulsed plasma deposited maleic anhydride thin films as supports for lipid bilayers. Langmuir
  32. (1992). Quantitative determination of molecular structure in multilayered thin films of biaxial and lower symmetry from photon spectroscopies. I. Reflection infrared vibrational spectroscopy.
  33. (1994). Reflectance infrared spectroscopic analysis of monolayer films at the air-water interface
  34. (2006). Soft lithographic patterning of supported lipid bilayers onto a surface and inside microfluidic channels. Lab Chip
  35. (2006). Solid supported lipid bilayers: From biophysical studies to sensor design.
  36. (1987). Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistry.
  37. Structural evolution of selfassembled alkanephosphate monolayers on TiO2.
  38. (1998). Structural studies of polymer-cushioned lipid bilayers.
  39. (1996). Supported membranes: scientific and practical applications. Science
  40. (1986). Supported planar membranes in studies of cell-cell recognition in the immune system.
  41. (1998). Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance.
  42. (2002). The polymersupported phospholipid bilayer: Tethering as a new approach to substrate-membrane stabilization. Biomacromolecules
  43. (2004). Vesicle adsorption and lipid bilayer formation on glass studied by atomic force microscopy. Langmuir
  44. (1979). Vibrational spectra and carbon–hydrogen stretching mode assignments for a series of N-alkyl carboxylic acids.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.