Skip to main content
Article thumbnail
Location of Repository

Force- and length-dependent catastrophe activities explain interphase microtubule organization in fission yeast

By Dietrich Foethke, Tatyana Makushok, Damian Brunner and François Nédélec

Abstract

The cytoskeleton is essential for the maintenance of cell morphology in eukaryotes. In fission yeast, for example, polarized growth sites are organized by actin, whereas microtubules (MTs) acting upstream control where growth occurs. Growth is limited to the cell poles when MTs undergo catastrophes there and not elsewhere on the cortex. Here, we report that the modulation of MT dynamics by forces as observed in vitro can quantitatively explain the localization of MT catastrophes in Schizosaccharomyces pombe. However, we found that it is necessary to add length-dependent catastrophe rates to make the model fully consistent with other previously measured traits of MTs. We explain the measured statistical distribution of MT–cortex contact times and re-examine the curling behavior of MTs in unbranched straight tea1Δ cells. Importantly, the model demonstrates that MTs together with associated proteins such as depolymerizing kinesins are, in principle, sufficient to mark the cell poles

Topics: Report
Publisher: Nature Publishing Group
OAI identifier: oai:pubmedcentral.nih.gov:2671915
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (2002). Roles of fission yeast tea1p in the localization of polarity factors and in organizing the microtubular cytoskeleton.
    2. (2006). Yeast kinesin-8 depolymerizes microtubules in a lengthdependent manner.
    3. This article is licensed under a Creative Commons AttributionNoncommercial-Share Alike 3.0 Licence. Modelling microtubule organization in fission yeast

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.