Skip to main content
Article thumbnail
Location of Repository

Substrate Binding in the FAD-Dependent Hydroxynitrile Lyase from Almond Provides Insight into the Mechanism of Cyanohydrin Formation and Explains the Absence of Dehydrogenation Activity†,‡

By Ingrid Dreveny, Aleksandra S. Andryushkova, Anton Glieder, Karl Gruber and Christoph Kratky
Topics:
Publisher: American Chemical Society
OAI identifier: oai:pubmedcentral.nih.gov:2669238
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (1999). 1.8 and 1.9 Å resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modeling substrate complexes. Acta Crystallogr.
    2. (1998). Aspects of the mechanism of catalysis of glucose oxidase: a docking, molecular mechanics and quantum chemical study.
    3. (1999). Atomic resolution structure of hydroxynitrile lyase from HeVea brasiliensis.
    4. (2004). Biocatalytic conversion of unnatural substrates by recombinant almond R-HNL isoenzyme 5.
    5. (2005). Carving the active site of almond R-HNL for increased enantioselectivity.
    6. (1984). Chemical modification of hydroxynitrile lyase by selective reaction of an essential cysteineSH group with alpha, beta-unsaturated propiophenones as pseudosubstrates.
    7. (2003). Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis.
    8. (2000). Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of betaD-glucose.
    9. (1993). Crystal structure of cholesterol oxidase complexed with a steroid substrate: implications for flavin adenine dinucleotide dependent alcohol oxidases.
    10. (2002). Crystal structure of hydroxynitrile lyase from Sorghum bicolor in complex with the inhibitor benzoic acid: a novel cyanogenic enzyme.
    11. (2004). Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase.
    12. (1998). Crystallography and NMR system (CNS): a software system for macromolecular structure determination. Acta Crystallogr.
    13. (1985). Cyanogenic compounds as protecting agents for organisms.
    14. (1978). D-hydroxynitrile lyase: involvement of the prosthetic flavin adenine dinucleotide in enzyme activity.
    15. (2001). Elucidation of the mode of substrate binding to hydroxynitrile lyase from HeVea brasiliensis.
    16. (2002). Flavoenzymes that catalyse reactions with no net redox change.
    17. (2000). Flavoenzymes: diverse catalysts with recurrent features.
    18. (1992). GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activities.
    19. (1996). Hydroxynitrile lyases of higher plants.
    20. (1996). Hydroxynitrile lyases: functions and properties.
    21. (1991). Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr.
    22. (1981). in The Biochemistry of Plants: A ComprehensiVe Treatise
    23. (1986). Isolation and characterization of multiple forms of mandelonitrile lyase from mature black cherry (Prunus serotina Ehrh.) seeds.
    24. (1979). Mechanism of catalysis by the flavoenzyme oxynitrilase.
    25. (1996). Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from HeVea brasiliensis.
    26. (2003). Mechanism of the reductive half-reaction in cellobiose dehydrogenase.
    27. (2001). Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin.
    28. (1997). Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases.
    29. (1996). Molecular cloning of the full-length cDNA of (S) -hydroxynitrile lyase from HeVea brasiliensis. Functional expression in Escherichia coli and Saccharomyces cereVisiae and identification of an active site residue.
    30. (1991). MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures.
    31. (2005). On the catalytic mechanism of choline oxidase.
    32. (1966). On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones.
    33. (2007). Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry.
    34. (1997). Processing of X-ray diffraction data collected in oscillation mode.
    35. (1997). Raster3D: photorealistic molecular graphics.
    36. (2006). Reaction geometry and thermostable variant of pyranose 2-oxidase from the white-rot fungus Peniophora sp.
    37. (2004). Reaction mechanism of hydroxynitrile lyases of the alpha/ beta-hydrolase superfamily: the three-dimensional structure of the transient enzyme-substrate complex certifies the crucial role of LYS236.
    38. (2003). Role of the flavin domain residues,
    39. (2006). Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme.
    40. (2006). Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase.
    41. (2001). Structure of hydroxynitrile lyase from Manihot esculenta in complex with substrates acetone and chloroacetone: implications for the mechanism of cyanogenesis.
    42. (1980). Studies on the kinetics of cyanohydrin synthesis and cleavage by the flavoenzyme oxynitrilase.
    43. (2003). Sub-atomic resolution crystal structure of cholesterol oxidase: what atomic resolution crystallography reveals about enzyme mechanism and the role of the FAD cofactor in redox activity.
    44. (2002). The active site of hydroxynitrile lyase from Prunus amygdalus: modeling studies provide new insights into the mechanism of cyanogenesis.
    45. (2004). The chemical mechanism of action of glucose oxidase from Aspergillus niger.
    46. (2001). The hydroxynitrile lyase from almond: a lyase that looks like an oxidoreductase.
    47. (2001). The presence of a hydrogen bond between asparagine 485 and the pi system of FAD modulates the redox potential in the reaction catalyzed by cholesterol oxidase.
    48. (1969). The reactivity of flavoproteins with sulfite. Possible relevance to the problem of oxygen reactivity.
    49. (1966). U ¨ber das flavinenzym Doxynitrilase.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.