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Expression of ricin B chain in Escherichia coli

By Khalid Hussain, Christopher Bowler, Lynne M. Roberts and J.Michael Lord


AbstractDNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form

Publisher: Published by Elsevier B.V.
Year: 1989
DOI identifier: 10.1016/0014-5793(89)80568-X
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