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Loss of the Synaptic Vesicle Protein SV2B Results in Reduced Neurotransmission and Altered Synaptic Vesicle Protein Expression in the Retina

By Catherine W. Morgans, Patricia Kensel-Hammes, James B. Hurley, Kimberly Burton, Rejean Idzerda, G. Stanley McKnight and Sandra M. Bajjalieh


The Synaptic Vesicle Protein 2 (SV2) family of transporter-like proteins is expressed exclusively in vesicles that undergo calcium-regulated exocytosis. Of the three isoforms expressed in mammals, SV2B is the most divergent. Here we report studies of SV2B location and function in the retina. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. In mice lacking SV2B, synaptic transmission at the synapse between photoreceptors and bipolar neurons was decreased, as evidenced by a significant reduction in the amplitude of the b-wave in electroretinogram recordings. Quantitative immunoblot analyses of whole eyes revealed that loss of SV2B was associated with reduced levels of synaptic vesicle proteins including synaptotagmin, VAMP, synaptophysin and the vesicular glutamate transporter V-GLUT1. Immunolabeling studies revealed that SV2B is detected in rod photoreceptor synaptic terminals where it is the primary isoform. Thus, SV2B contributes to the modulation of synaptic vesicle exocytosis and plays a significant role in regulating synaptic protein content

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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  1. (1999). Abnormal neurotransmission in mice lacking synaptic vesicle protein 2A (SV2A).
  2. (2004). An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size.
  3. (1999). AP-2 recruitment to synaptotamin stimulated by tyrosine-based endocytic motifs.
  4. (1993). Brain contains two forms of synaptic vesicle protein 2.
  5. (2003). Differential distribution and developmental expression of synaptic vesicle protein 2 isoforms in the mouse retina.
  6. (1994). Differential expression of Synaptic Vesicle Protein 2 (SV2) isoforms.
  7. (2003). Distribution of the presynaptic calcium sensors, synaptotagmin I/II and synaptotagmin III, in the goldfish and rodent retinas.
  8. (2007). Gbeta5 is required for normal light responses and morphology of retinal ON-bipolar cells.
  9. (1981). Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue.
  10. (1985). Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells.
  11. (1996). Isoform-specific, calciumregulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin.
  12. (2003). Molecular mechanisms of active zone function.
  13. (2001). Multiple phosphorylation of rhodopsin and the in vivo chemistry underlying rod photoreceptor dark adaptation.
  14. (2005). Photoreceptor calcium channels: insight from night blindness.
  15. (2001). SNARE function analyzed in synaptobrevin/VAMP knockout mice.
  16. Sudhof TC (2009) SV2 renders primed synaptic vesicles competent for Ca2+ -induced exocytosis.
  17. (2001). SV2 modulates the size of the readily releasable pool of secretory vesicles.
  18. (1992). SV2, a brain synaptic vesicle protein homologous to bacterial transporters.
  19. (1999). SV2A and SV2B function as redundant Ca2+ regulators in neurotransmitter release.
  20. (2005). SV2A and SV2C contain a unique synaptotagmin-binding site.
  21. (2004). SV2B regulates synaptotagmin 1 by direct interaction.
  22. (1998). SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles.
  23. (2001). Synaptic vesicle alterations in rod photoreceptors of synaptophysin-deficient mice.
  24. (2006). Synaptic vesicle protein 2 enhances release probability at quiescent synapses.
  25. (1998). Synaptic vesicle protein SV2B, but not SV2A, is predominantly expressed and associated with microvesicles in rat pinealocytes.
  26. (2002). Synaptotagmin: a Ca(2+) sensor that triggers exocytosis?
  27. (2007). The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling.
  28. (2003). The presynaptic active zone protein bassoon is essential for photoreceptor ribbon synapse formation in the retina.
  29. (1992). The synaptic vesicle protein SV2 is a novel type of transmembrane transporter.
  30. (2004). The synaptic vesicle protein SV2A is the binding site for the antiepileptic drug levetiracetam.
  31. (1996). The Vo sector of the V-ATPase, synaptobrevin, and synaptophysin are assoicated on synaptic vesicles in a Triton X-100-resistant, freeze-thawing sensitive, complex.
  32. (2008). UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes.
  33. (1994). Vesicle-associated membrane protein and synaptophysin are associated on the synaptic vesicle.

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