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Label-free electrical quantification of the dielectrophoretic response of DNA

By Anja Henning, Jörg Henkel, Frank F Bier and Ralph Hölzel


A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 μL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible

Topics: Research Article
Publisher: BioMed Central
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Provided by: PubMed Central
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    1. (1990). (page number not for citation purposes)
    2. (2003). AC electrokinetics: colloids and nanoparticles Baldock, Research Studies Press;
    3. (2005). Anal Bioanal Chem
    4. (1999). Appl Phys
    5. (2004). Bier FF: AIP Conf Proc
    6. (1999). Biochim Biophys Acta
    7. (1969). Birshtein TM: Biopolymers
    8. (2002). Castellanos A: Phys Rev E
    9. (1978). Dielectrophoresis Cambridge,
    10. (2001). DW: Molecular cloning: A laboratory manual
    11. (2002). Engh G van den: Electrophoresis
    12. (2003). FF: IEE Proc Nanobiotechnol
    13. (2005). FF: Phys Rev Lett
    14. (2005). Hamers RJ: Nanolett
    15. (2008). Hinterdorfer P: Nano Today
    16. (2002). Hölzel R: AIP Conf Proc
    17. (2004). MF: Nucl Acid Res
    18. (2006). Nature
    19. (1993). Phys D: Appl Phys
    20. (2004). PJ: Biosens Bioelectron
    21. (2007). Rito-Palomares M: Electrophoresis
    22. (1993). Science
    23. (2002). Seeman NC: Proc Nat Acad Sci (USA)
    24. (1994). Shinohara T:
    25. (1998). WB: Electronics Lett
    26. (2006). WM: Curr Appl Phys

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