Skip to main content
Article thumbnail
Location of Repository

Label-free electrical quantification of the dielectrophoretic response of DNA

By Anja Henning, Jörg Henkel, Frank F Bier and Ralph Hölzel

Abstract

A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 μL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible

Topics: Research Article
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:2666632
Provided by: PubMed Central

Suggested articles

Citations

  1. (1990). (page number not for citation purposes)
  2. (2003). AC electrokinetics: colloids and nanoparticles Baldock, Research Studies Press;
  3. (2005). Anal Bioanal Chem
  4. (1999). Appl Phys
  5. (2004). Bier FF: AIP Conf Proc
  6. (1999). Biochim Biophys Acta
  7. (1969). Birshtein TM: Biopolymers
  8. (2002). Castellanos A: Phys Rev E
  9. (1978). Dielectrophoresis Cambridge,
  10. (2001). DW: Molecular cloning: A laboratory manual
  11. (2002). Engh G van den: Electrophoresis
  12. (2003). FF: IEE Proc Nanobiotechnol
  13. (2005). FF: Phys Rev Lett
  14. (2005). Hamers RJ: Nanolett
  15. (2008). Hinterdorfer P: Nano Today
  16. (2002). Hölzel R: AIP Conf Proc
  17. (2004). MF: Nucl Acid Res
  18. (2006). Nature
  19. (1993). Phys D: Appl Phys
  20. (2004). PJ: Biosens Bioelectron
  21. (2007). Rito-Palomares M: Electrophoresis
  22. (1993). Science
  23. (2002). Seeman NC: Proc Nat Acad Sci (USA)
  24. (1994). Shinohara T:
  25. (1998). WB: Electronics Lett
  26. (2006). WM: Curr Appl Phys

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.